FluoroNanogold™ Conjugates

Fluorescent labels PLUS Nanogold® - covalently bound
Make your target visible in almost any microscope !

See your target's fluorescent label as before
PLUS the Nanogold label in light microscopes, even EM

Put your targets back into their cellular context!
Correlate between confocal, light microscopy and EM

• Available in convenient conjugates to secondary antibodies (or biotin/streptavidin)
• Uses standard secondary antibody labeling protocols
• Easily grow the Nanogold® particle to any size after labeling
  with our Gold and Silver Enhancers
  -for low-mag applications, or even to the naked eye
  

"FNG [FluoroNanogold™] is a probe containing two different markers; it opens the possibility of imaging the same sample at both the optical and the EM level. Thus, it is easy to perform a multimodal investigation, either on different cells or on the same cell visualized by correlative microscopy."

Cheutin, T.; Sauvage, C.; Tchélidzé, P, O'Donohue, M. F.; Kaplan, H.; Beorchia, A., and Ploton, D.: Visualizing macromolecules with FluoroNanogold: from photon microscopy to electron tomography. Methods Cell Biol., 79, 559–574 (2007). [This is an entire chapter on FluoroNanogold, providing a detailed and well illustrated write-up of techniques for combined confocal fluorescence microscopic labeling and electron tomography.]

FluoroNanogold™ Conjugates 1.4 nm Nanogold® PLUS fluorescent dye: Secondary antibody and streptavidin conjugates Label for multiple microscopes in one step! For confocal, EM, LM, blotting and diagnostics. Need rapid, precise enhancement? Try our Silver Enhancers and Gold Enhancers Custom conjugation is also available, to primary antibodies, peptides, small molecules, or other molecules.   FluoroNanogold™ Secondary Antibody Conjugates Anti-Mouse #7001-1ML Fluorescein - FluoroNanogold™ IgG Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7001-0.5ML Fluorescein - FluoroNanogold™ IgG Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7002-1ML Fluorescein - FluoroNanogold™ Fab' Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7002-0.5ML Fluorescein - FluoroNanogold™ Fab' Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7201-1ML Alexa Fluor® 488 - FluoroNanogold™ IgG Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7201-0.5ML Alexa Fluor® 488 - FluoroNanogold™ IgG Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7202-1ML Alexa Fluor® 488 - FluoroNanogold™ Fab' Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7202-0.5ML Alexa Fluor® 488 - FluoroNanogold™ Fab' Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7301-1ML Alexa Fluor ® 594 - FluoroNanogold™ IgG Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7301-0.5ML Alexa Fluor® 594 - FluoroNanogold™ IgG Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7302-1ML Alexa Fluor® 594 - FluoroNanogold™ Fab' Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7302-0.5ML ALexa Fluor® 594 - FluoroNanogold™ Fab' Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7401-1ML Alexa Fluor® 546 - FluoroNanogold™ IgG Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7401-0.5ML Alexa Fluor® 546 - FluoroNanogold™ IgG Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7402-1ML Alexa Fluor® 546 - FluoroNanogold™ Fab' Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7402-0.5ML Alexa Fluor® 546 - FluoroNanogold™ Fab' Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7501-1ML Alexa Fluor® 647 - FluoroNanogold™ IgG Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7501-0.5ML Alexa Fluor® 647 - FluoroNanogold™ IgG Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7502-1ML Alexa Fluor® 647 - FluoroNanogold™ Fab' Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7502-0.5ML Alexa Fluor® 647 - FluoroNanogold™ Fab' Goat anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) Anti Rabbit #7003-1ML Fluorescein - FluoroNanogold™ IgG Goat anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7003-0.5ML Fluorescein - FluoroNanogold™ IgG Goat anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7004-1ML Fluorescein - FluoroNanogold™ Fab' Goat anti-Rabbit IgG (H+L) (min X Hu Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7004-0.5ML Fluorescein - FluoroNanogold™ Fab' Goat anti-Rabbit IgG (H+L) (min X Hu Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7203-1ML Alexa Fluor® 488 - FluoroNanogold™ IgG Goat anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7203-0.5ML Alexa Fluor® 488 - FluoroNanogold™ IgG Goat anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7204-1ML Alexa Fluor® 488 - FluoroNanogold™ Fab' Goat anti-Rabbit IgG (H+L) (min X Hu Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7204-0.5ML Alexa Fluor® 488 - FluoroNanogold™ Fab' Goat anti-Rabbit IgG (H+L) (min X Hu Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7303-1ML Alexa Fluor® 594 - FluoroNanogold™ IgG Goat anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7303-0.5ML Alexa Fluor® 594 - FluoroNanogold™ IgG Goat anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7304-1ML Alexa Fluor® 594 - FluoroNanogold™ Fab' Goat anti-Rabbit IgG (H+L) (min X Hu Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7304-0.5ML Alexa Fluor® 594 - FluoroNanogold™ Fab' Goat anti-Rabbit IgG (H+L) (min X Hu Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7403-1ML Alexa Fluor® 546 - FluoroNanogold™ IgG Goat anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7403-0.5ML Alexa Fluor® 546 - FluoroNanogold™ IgG Goat anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7404-1ML Alexa Fluor® 546 - FluoroNanogold™ Fab' Goat anti-Rabbit IgG (H+L) (min X Hu Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7404-0.5ML Alexa Fluor® 546 - FluoroNanogold™ Fab' Goat anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7503-1ML Alexa Fluor® 647 - FluoroNanogold™ IgG Goat anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7503-0.5ML Alexa Fluor® 647 - FluoroNanogold™ IgG Goat anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7504-1ML Alexa Fluor® 647 - FluoroNanogold™ Fab' Goat anti-Rabbit IgG (H+L) (min X Hu Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7504-0.5ML Alexa Fluor® 647 - FluoroNanogold™ Fab' Goat anti-Rabbit IgG (H+L) (min X Hu Sr Prot) 0.5 mL $366 Qty: Code (Optional) Anti Goat #7205-1ML Alexa Fluor® 488 - FluoroNanogold™ IgG Rabbit anti-Goat IgG (H+L) (min X Hu Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7205-0.5ML Alexa Fluor® 488 - FluoroNanogold™ IgG Rabbit anti-Goat IgG (H+L) (min X Hu Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7206-1ML Alexa Fluor® 488 - FluoroNanogold™ Fab' Rabbit anti-Goat IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7206-0.5ML Alexa Fluor® 488 - FluoroNanogold™ Fab' Rabbit anti-Goat IgG (H+L) (min X Hu Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7505-1ML Alexa Fluor® 647 - FluoroNanogold™ IgG Rabbit anti-Goat IgG (H+L) (min X Hu Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7505-0.5ML Alexa Fluor® 647 - FluoroNanogold™ IgG Rabbit anti-Goat IgG (H+L) (min X Hu Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7506-1ML Alexa Fluor® 647 - FluoroNanogold™ Fab' Rabbit anti-Goat IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7506-0.5ML Alexa Fluor® 647 - FluoroNanogold™ Fab' Rabbit anti-Goat IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 0.5 mL $366 Qty: Code (Optional) Anti Rat #7207-1ML Alexa Fluor® 488 - FluoroNanogold™ IgG Goat anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7207-0.5ML Alexa Fluor® 488 - FluoroNanogold™ IgG Goat anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7208-1ML Alexa Fluor® 488 - FluoroNanogold™ Fab' Goat anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7208-0.5ML Alexa Fluor® 488 - FluoroNanogold™ Fab' Goat anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7507-1ML Alexa Fluor® 647 - FluoroNanogold™ IgG Goat anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7507-0.5ML Alexa Fluor® 647 - FluoroNanogold™ IgG Goat anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7508-1ML Alexa Fluor® 647 - FluoroNanogold™ Fab' Goat anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7508-0.5ML Alexa Fluor® 647 - FluoroNanogold™ Fab' Goat anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) 0.5 mL $366 Qty: Code (Optional) Anti Sheep #7250-1ML Alexa Fluor® 488 - FluoroNanogold™ IgG Rabbit anti-Sheep IgG (H+L) (min X Hu Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7250-0.5ML Alexa Fluor® 488 - FluoroNanogold™ IgG Rabbit anti-Sheep IgG (H+L) (min X Hu Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7251-1ML Alexa Fluor® 488 - FluoroNanogold™ Fab' Rabbit anti-Sheep IgG (H+L) 1.0 mL $609 Qty: Code (Optional) #7251-0.5ML Alexa Fluor® 488 - FluoroNanogold™ Fab' Rabbit anti-Sheep IgG (H+L) (min X Hu Sr Prot) 0.5 mL $366 Qty: Code (Optional) Anti Human #7252-1ML Alexa Fluor® 488 - FluoroNanogold™ IgG Goat anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7252-0.5ML Alexa Fluor® 488 - FluoroNanogold™ IgG Goat anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7253-1ML Alexa Fluor® 488 - FluoroNanogold™ Fab' Goat anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7253-0.5ML Alexa Fluor® 488 - FluoroNanogold™ Fab' Goat anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) 1.0 mL $366 Qty: Code (Optional) #7552-1ML Alexa Fluor® 647 - FluoroNanogold™ IgG Goat anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7552-0.5ML Alexa Fluor® 647 - FluoroNanogold™ IgG Goat anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) 0.5 mL $366 Qty: Code (Optional) #7553-1ML Alexa Fluor® 647 - FluoroNanogold™ Fab' Goat anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) 1.0 mL $609 Qty: Code (Optional) #7553-0.5ML Alexa Fluor® 647 - FluoroNanogold™ Fab' Goat anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) 0.5 mL $366 Qty: Code (Optional) Anti Guinea Pig #7054-1ML Fluorescein - FluoroNanogold™ IgG Goat anti-Guinea Pig IgG (H+L) 1.0 mL $609 Qty: Code (Optional) #7054-0.5ML Fluorescein - FluoroNanogold™ IgG Goat anti-Guinea Pig IgG (H+L) 0.5 mL $366 Qty: Code (Optional) #7055-1ML Fluorescein - FluoroNanogold™ Fab' Goat anti-Guinea Pig IgG (H+L) 1.0 mL $609 Qty: Code (Optional) #7055-0.5ML Fluorescein - FluoroNanogold™ Fab' Goat anti-Guinea Pig IgG (H+L) 0.5 mL $366 Qty: Code (Optional) #7354-1ML Alexa Fluor® 594 - FluoroNanogold™ IgG Goat anti-Guinea Pig IgG (H+L) 1.0 mL $609 Qty: Code (Optional) #7354-0.5ML Alexa Fluor® 594 - FluoroNanogold™ IgG Goat anti-Guinea Pig IgG (H+L) 0.5 mL $366 Qty: Code (Optional) #7355-1ML Alexa Fluor® 594 - FluoroNanogold™ Fab' Goat anti-Guinea Pig IgG (H+L) 1.0 mL $609 Qty: Code (Optional) #7355-0.5ML Alexa Fluor® 594 - FluoroNanogold™ Fab' Goat anti-Guinea Pig IgG (H+L) 0.5 mL $366 Qty: Code (Optional) #7554-1ML Alexa Fluor® 647 - FluoroNanogold™ IgG Goat anti-Guinea Pig IgG (H+L) 1.0 mL $609 Qty: Code (Optional) #7554-0.5ML Alexa Fluor® 647 - FluoroNanogold™ IgG Goat anti-Guinea Pig IgG (H+L) 0.5 mL $366 Qty: Code (Optional) #7555-1ML Alexa Fluor® 647 - FluoroNanogold™ Fab' Goat anti-Guinea Pig IgG (H+L) 1.0 mL $609 Qty: Code (Optional) #7555-0.5ML Alexa Fluor® 647 - FluoroNanogold™ Fab' Goat anti-Guinea Pig IgG (H+L) 0.5 mL $366 Qty: Code (Optional) FluoroNanogold™ - Streptavidin #7016-1ML Fluorescein - FluoroNanogold™ Streptavidin 1.0 mL $679 Qty: Code (Optional) #7016-0.5ML Fluorescein - FluoroNanogold™ Streptavidin 0.5 mL $406 Qty: Code (Optional) #7216-1ML Alexa Fluor® 488 - FluoroNanogold™ Streptavidin 1.0 mL $679 Qty: Code (Optional) #7216-0.5ML Alexa Fluor® 488 - FluoroNanogold™ Streptavidin 0.5 mL $406 Qty: Code (Optional) #7316-1ML Alexa Fluor® 594 - FluoroNanogold™ Streptavidin 1.0 mL $679 Qty: Code (Optional) #7316-0.5ML Alexa Fluor® 594 - FluoroNanogold™ Streptavidin 0.5 mL $406 Qty: Code (Optional) #7416-1ML Alexa Fluor® 546 - FluoroNanogold™ Streptavidin 1.0 mL $679 Qty: Code (Optional) #7416-0.5ML Alexa Fluor® 546 - FluoroNanogold™ Streptavidin 0.5 mL $406 Qty: Code (Optional) #7516-1ML Alexa Fluor® 647 - FluoroNanogold™ Streptavidin 1.0 mL $679 Qty: Code (Optional) #7516-0.5ML Alexa Fluor® 647 - FluoroNanogold™ Streptavidin 0.5 mL $406 Qty: Code (Optional)

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Learn more about our FluoroNanogold™ products...
Available in convenient conjugates to secondary antibodies, or streptavidin:

• Features and applications
• Specific properties of conjugates:
  • Fluorescein FluoroNanogold™
  • Alexa Fluor®* 488 FluoroNanogold™
  • Alexa Fluor®* 594 FluoroNanogold™
  • Alexa Fluor®* 546 FluoroNanogold™
  • Alexa Fluor® 647 FluoroNanogold™ for super-resolution!
• Custom FluoroNanogold™ Conjugates

• Featured paper: Sato et al. (2013) used FluoroNanogold™ to explore the mechanism behind cellular dynamics, using correlative microscopy with fluorescence and the new Atmospheric SEM (ASEM). Check out their great images!
• References: Papers published that use FluoroNanogold™
• Instructions and Protocols
• Technical Help
• MSDS

By combining gold and fluorescence into one immunoprobe, the same specimen may be imaged using both fluorescence microscopy (e.g., with a confocal microscope) and at the ultrastructural level by electron microscopy.

FluoroNanogold™is now available with Alexa Fluor®* 647, 546, 488 or 594, giving you the benefits of brighter fluorescence, reduced photobleaching, and compatibility with a wider pH range, or in its original formulation with fluorescein as the fluorophore.

Unlike colloidal golds which quench fluorescence, tiny Nanogold® lets the fluorescence shine through. All components are covalently attached to ensure stability and long shelf life. Fab' antibody fragments are much smaller probes than IgG conjugates, and have shown excellent penetration into cells and nuclei. FluoroNanogold™conjugates are chromatographically purified to eliminate any aggregates, free gold or unattached fluorescent molecules.

• Unprecedented correlation between fluorescence and EM data.
• Single labeling procedure means less chance for specimen perturbation.
• Choice of new fluorophores for brighter fluorescence, lower background and multicolor labeling.
• Same excellent penetration as found with Nanogold®: much better than 5 and 10 nm colloidal gold probes.
• FluoroNanogold™ probes are smaller than IgG conjugates (we use Fab' fragments).
• Covalent coupling of both labels gives stability and long shelf life.
• Conjugates are stable and fluorescent at a wide range of pH and ionic strengths.
  

Applications

• Correlative fluorescence and electron microscopy (References)
• Check your labeling by fluorescence before processing for EM
• Monitor a dynamic process by fluorescence to determine when to fix and process for EM.
• Differentiate different targets using different fluorophores.

Alexa Fluor®* 647 FluoroNanogold™ 2-in-1 immunolabeling for Super-Res!

Simultaneously label for Super-Res AND TEM / light microscopes in a single, standard immunolabeling procedure Each secondary antibody / Fab' includes TWO labels --Covalently bound for stability and long working times: Fluorescent dye for imaging in Super-Resolution Nanogold® particles mark your target for TEM, or light microscopy Finally-- Put your Super-Res images into cellular context! Reveal your fluorescent labeling in traditional microscopes, where you can see the surrounding cell structures! TEM, light microscope, phase contrast, etc. Adaptable to any resolution, for ultimate flexibility in correlative studies Easy enhancement kits grow the Nanogold® as big as you like

Fluorescent labeling of Alexa Fluor® 647 and Nanogold® - Fab' tertiary probe. The specimen is a slide from the NOVA Lite ANA HEp-2 test, an indirect immunofluorescent test system for the screening and semi-quantitative determination of anti-nuclear antibodies (ANA) in human serum (see ). The slide was stained using positive pattern control human sera, a Mouse anti-Human secondary antiboidy, and combined Alexa Fluor® 647 and Nanogold® - Fab' tertiary probe. Specimens were washed with PBS (30 minutes) between each step, then blocked by the addition of 7 % nonfat dried milk to the tertiary antibody solution (original magnification 400 X).

Alexa Fluor® 647 FluoroNanogold™ -- IgG and Fab' Conjugates   Product information   Material Safety Data Sheet

Alexa Fluor® 647 FluoroNanogold™ -- Streptavidin   Product information   Material Safety Data Sheet

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Alexa Fluor®* 488 FluoroNanogold™

• Increased fluorescence signal and higher quantum yield - ideal for scarce targets or dynamic systems where exposure needs to be restricted.
• Fluorescence remains high and consistent across wider pH range.
• Improved solubility means reduced non-specific interactions, lower background and higher signal-to-noise ratios.
• Uses fluorescein filter sets.
• Available in 1 mL or affordable 0.5 mL sizes.

Left: Structure of Alexa Fluor® 488 and Nanogold® - Fab', showing covalent attachment of components.

Right: Fluorescent staining obtained using combined combined Alexa Fluor® 488 and Nanogold® - Fab' tertiary probe. The specimen is a slide from the NOVA Lite ANA HEp-2 test, an indirect immunofluorescent test system for the screening and semi-quantitative determination of anti-nuclear antibodies (ANA) in human serum (see ). The slide was stained using positive pattern control human sera, a Mouse anti-Human secondary antiboidy, and combined Alexa Fluor® 488 and Nanogold® - Fab' tertiary probe. Specimens were washed with PBS (30 minutes) between each step, then blocked by the addition of 7 % nonfat dried milk to the tertiary antibody solution (original magnification 400 X).

Alexa Fluor®* 546 FluoroNanogold™

• Increased fluorescence signal and higher quantum yield - ideal for scarce targets or dynamic systems where exposure needs to be restricted.
• Fluorescence remains high and consistent across wider pH range.
• Improved solubility means reduced non-specific interactions, lower background and higher signal-to-noise ratios.
• Uses fluorescein filter sets.
• Available in 1 mL or affordable 0.5 mL sizes.

Alexa Fluor®* 594 FluoroNanogold™

• Use for multiple labeling: distinguish a FluoroNanogold™-labeled target from a second target labeled with fluorescein, Alexa Fluor® 488, green fluorescent protein, or other fluorophores.
• Uses Texas Red filter sets.
• Available in 1 mL or affordable 0.5 mL sizes.

Localization of caveolin-1a in ultrathin cryosection of human placenta using a new FNG; caveolin 1 alpha is primarily located to caveolae in placental endothelial cells. One-to-one correspondence is found between fluorescent spots and caveola labeled with gold particles (right). Ultrathin cryosections collected on formvar film-coated nickel EM grids were incubated with chicken anti-human caveolin-1a IgY for 30 min at 37°C, then with biotinylated goat anti-chicken F(ab)₂ (13 mg/ml) for 30 min at 37°C, then stained with ALEXA-594 FluoroNanogold™-Streptavidin (1:50 dilution) for 30 min at room temperature. Non-specific sites on cryosections were blocked with 1% milk - 5% fetal bovine serum-PBS for 30 minutes at room temperature (figure courtesy of T. Takizawa, Ohio State University, Columbus, OH).

Structure of Alexa Fluor®* 594 FluoroNanogold™Fab' and Streptavidin conjugates, showing covalent attachment.

Fluorescein FluoroNanogold™

Fluorescein is the most widely used fluorophore; fluorescein FluoroNanogold™conjugates combine fluorescein and Nanogold® in a single convenient, stable probe. correlate fluorescein fluorescence staining with brightfield light microscopy and electron microscopy using a single convenient immunostaining procedure. Check labeling by fluorescence before EM processing. Available in 1 mL or affordable 0.5 mL sizes.

FluoroNanogold™Staining of Microtubules

Immunolocalization of microtubules with Fluorescein FluoroNanogold™-Fab' in the same human monocyte visualized by various microscopies: (A) Fluorescence; (B) Phase; (C) DIC, and (D) Electron Microscopy (with silver enhancement). (Micrographs courtesy of Dr. J. M. Robinson and Dr. D. Vandré, Ohio State University).

Custom Labeling

We can also create custom conjugates, using your own primary antibody (IgG or Fab'), proteins, peptides, lectins, and other molecules. Please use our Custom Synthesis Form to let us know your needs, or drop us a line by phone or email at tech@nanoprobes.com.

* Alexa Fluor is a registered trademark of Life Technologies, Inc.