References: FluoroNanogold™

• Protocols
• Correlative microscopy
• In situ Hybridization
• Silver and gold enhancement

Protocols

• Viall CA, Holloway H, Chen Q, Stone PR, Chamley LW, Development of a simple, cost-effective, semi-correlative light and electron microscopy method to allow the immunoelectron localisation of non-uniformly distributed placental proteins, Placenta (2014), doi: 10.1016/j.placenta.2014.01.005.
• Takizawa, T., and Robinson, J. M.: Correlative fluorescence and transmission electron microscopy in tissues. Methods Cell Biol., 111, 37-57 (2012).
• Salio C, Lossi L, Merighi A. Combined light and electron microscopic visualization of neuropeptides and their receptors in central neurons. Methods Mol Biol. 2011;789:57-71.
  • "We describe here a preembedding procedure employing the Fluoronanogold™ reagent...made visible under the electron microscope by a gold intensification procedure. This technique permits correlative fluorescence and electron microscopy observations, providing a very useful tool for the study of neuronal connectivity. Moreover, the Fluoronanogold™ procedure can be combined with conventional postembedding immunogold techniques in multiple labeling studies."
• Robinson, J. M., and Takizawa, T.: Correlative fluorescence and electron microscopy in tissues: immunocytochemistry. J. Microsc., 235, 259-272 (2009).
• Cheutin, T.; Sauvage, C.; Tchélidzé, P, O'Donohue, M. F.; Kaplan, H.; Beorchia, A., and Ploton, D.: Visualizing macromolecules with FluoroNanogold: from photon microscopy to electron tomography. Methods Cell Biol., 79, 559–574 (2007).
  • An entire chapter on FluoroNanogold; provides a detailed and well illustrated write-up of their technique for combined confocal fluorescence microscopic labeling and electron tomography in thick sections.
• Takizawa, T., and Robinson, J. M.: Correlative Microscopy of Ultrathin Cryosections is a Powerful Tool for Placental Research. Placenta, 24, 557-565 (2003).

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Correlative Microscopy

1. Francesco Ferrini, Arianna Russo, Chiara Salio. Fos and pERK immunoreactivity in spinal cord slices: comparative analysis of in vitro models for testing putative antinociceptive molecules. Annals of Anatomy - Anatomischer Anzeiger, 31 December 2013.
2. A. Iván Hernández, William C. Oxberry, John F. Crary, Suzanne S. Mirra and Todd Charlton Sacktor. Cellular and subcellular localization of PKMζ. Phil. Trans. R. Soc. B 2014 369, 20130140, published 2 December 2013.
3. Ye Yuan, Si Chen, Tatjana Paunesku, Sophie Charlotte Gleber, William C. Liu, Caroline B. Doty, Rachel Mak, Junjing Deng, Qiaoling Jin, Barry Lai, Keith Brister, Claus Flachenecker, Chris Jacobsen, Stefan Vogt, and Gayle E. Woloschak. Epidermal Growth Factor Receptor Targeted Nuclear Delivery and High Resolution Whole Cell X-ray Imaging of Fe3O4@TiO2 Nanoparticles in Cancer Cells. ACS Nano, Publication Date (Web): November 12, 2013.
   • A method using FluoroNanogold™ in the neutral comet assay, which can be used to measure double-strand DNA breaks over a range of 50 to 10,000 breaks per cell.
4. Hirano, K.; Kinoshita, T.; Uemura, T.; Motohashi, H.; Watanabe, Y.; Ebihara, T.; Nishiyama, H.; Sato, M.; Suga, M.; Maruyama, Y.; Tsuji, N. M.; Yamamoto, M.; Nishihara, S., and Sato, C.: Electron microscopy of primary cell cultures in solution and correlative optical microscopy using ASEM. Ultramicroscopy (2013 Oct 22): in press.
   • The Sato lab explores the mechanism behind cellular dynamics, using correlative microscopy with fluorescence and the atmospheric scanning electron microscope (ASEM). The group used the dual-modality probe FluoroNanogold™ to label structures of interest with both a fluorescent probe and a tightly bound gold immunolabel for EM visualization. GoldEnhance™ was used to enlarge the probes with gold, making ultrastructures even more easily visible by backscatter detection in the SEM.
5. Takizawa, T., and Robinson, J. M.: Correlative fluorescence and transmission electron microscopy in tissues. Methods Cell Biol., 111, 37-57 (2012).
6. Jiao, W.; Shupliakov, A., and Shupliakov, O.: A semi-correlative technique for the subcellular localization of proteins in Drosophila synapses. J. Neurosci. Methods, 185, 273-279 (2010).
7. Robinson, J. M., and Takizawa, T.: Correlative fluorescence and electron microscopy in tissues: immunocytochemistry. J. Microsc., 235, 259-272 (2009).
8. Cheutin, T.; Sauvage, C.; Tchélidzé, P, O'Donohue, M. F.; Kaplan, H.; Beorchia, A., and Ploton, D.: Visualizing macromolecules with FluoroNanogold: from photon microscopy to electron tomography. Methods Cell Biol., 79, 559–574 (2007).
   • An entire chapter on FluoroNanogold; provides a detailed and well illustrated write-up of their technique for combined confocal fluorescence microscopic labeling and electron tomography in thick sections.
9. Takizawa, T., and Robinson, J. M.: Correlative Microscopy of Ultrathin Cryosections is a Powerful Tool for Placental Research. Placenta, 24, 557-565 (2003).
10. Humbel, B. M.; De Jong, M. D. M.; Müller, W. H., and Verkleij, A. J.: Pre-embedding immunolabeling for electron microscopy: An evaluation of permeabilization methods and markers. Microsc. Res. Tech., 42, 43-58 (1998).
11. Keohane, E. M.; Orr, G. A.;Takvorian, P. M.;Cali, A.; Tanowitz, H. B.; Wittner, M., and Weiss, L. M.: Polar tube proteins of Microsporida of the family Encephalitozoonidae; J. Euk. Microbiol., 46, 1-5 (1999).
12. Powell, R. D., and Hainfeld, J. F.: Combined Fluorescent and Gold Probes for Microscopic and Morphological Investigations. In Gold and Silver Staining: Techniques in Molecular Morphology, (G. W. Hacker and J. Gu, Eds.), CRC Press, Boca Raton, FL; pp. 107-118 (2002).
13. Powell, R. D.; Halsey, C. M. R., and Hainfeld, J. F.: Combined fluorescent and gold immunoprobes: Reagents and methods for correlative light and electron microscopy. Microsc. Res. Tech., 42, 2-12 (1998).
14. Powell, R. D.; Halsey, C. M. R.; Gutierrez, E.; Hainfeld, J. F., and Furuya, F. R.: Dual-labeled probes for fluorescence and electron microscopy. Proc. 56^th Ann. Mtg., Micros. Soc. Amer.; Bailey, G. W.; Alexander, K. B.; Jerome, W. G.; Bond, M. G., and McCarthy, J. J., Eds.; Springer, New York, NY, 1998, 992-993.
    • See the complete paper
15. Powell, R. D.; Halsey, C. M. R.; Spector, D. L.; Kaurin, S. L.; McCann, J.;, and Hainfeld, J. F. A covalent fluorescent-gold immunoprobe: "simultaneous" detection of a pre-mRNA splicing factor by light and electron microscopy. J. Histochem. Cytochem., 45, 947-956 (1997).
    • Reprint (PDF) from JHC Online.
    • See the abstract from JHC Online.
16. Powell, R. D.; Hainfeld, J. F.; Halsey, C. M. R.; Spector, D. L.; Kaurin, S.; McCann, J.; Craig, R.; Fay, F. S., and McNamara, K. E.: Large cluster and combined fluorescent and gold immunoprobes. In Proc 54^th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, J. M. Corbett, R. V. W. Dimlich, J. R. Michael and N. J., Zaluzec (Eds.). San Francisco Press, San Francisco, CA, pp. 892-893 (1996).
    • See the complete paper
17. Powell, R.D., Hainfeld, J.F., Churchill, M.E.A., and Belmont, A.S.I. Combined fluorescent and gold nucleic acid probes. In G. Bailey and A.J. Garratt-Reed (Eds.). Proc 52^nd Ann. Mtg. Micros. Soc. Amer., San Francisco Press, pp.176-177 (1994).
    • See the complete paper
18. Robinson, J. M.; Takizawa, T.: Biological labeling and correlative microscopy; Proc. 57^th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 474-475.
19. Robinson, J. M.; Takizawa, T., and Vandré, D. D.: Applications of gold cluster compounds in immunocytochemistry and correlative microscopy: comparison with colloidal gold. J. Microsc., 199, 163-79 (2000).
20. Robinson, J. M.; Takizawa, T., and Vandré: Enhanced immunolabeling efficiency using ultrasmall immunogold probes: Immunocytochemistry J. Histochem. Cytochem., 48, 487-492 (2000).
    • Abstract (from JHC Online).
21. Robinson, J. M.; Takizawa, T.; Vandré, D. D., and Burry, R. W.: Ultrasmall immunogold particles: important probes for immunocytochemistry; Microsc. Res. Tech., 42, 13-23 (1998).
22. Robinson, J. M.: FluoroNanogold: an efficient labeling reagent for immunocytochemistry. Proc. 56^th Ann. Mtg., Micros. Soc. Amer.; Bailey, G. W.; Alexander, K. B.; Jerome, W. G.; Bond, M. G., and McCarthy, J. J., Eds.; Springer, New York, NY, 1998, 990-991.
23. Robinson, J. M., and Vandré, D. D. Efficient immunocytochemical labeling of leukocyte microtubules with FluoroNanogold: An important tool for correlative microscopy. J. Histochem. Cytochem., 45, 631-642 (1997).
    • Reprint (PDF) from JHC Online.
    • See the abstract from JHC Online.
24. Takeuchi, S.; Takagishi, Y.; Yasui, K.; Murata, Y.; Toyama, J., and Kodama, I.: Voltage-gated K(+)Channel, kv4.2, localizes predominantly to the transverse-axial tubular system of the rat myocyte J. Mol. Cell.Cardiol., 32, 1361-1369 (2000).
    • Abstract (from the Journal of Molecular and Cellular Cardiology).
25. Takizawa, T., and Robinson, J. M.: Analysis of antiphotobleaching reagents for use with FluoroNanogold in correlative microscopy J. Histochem. Cytochem., 48, 433-436 (2000).
    • Abstract (from JHC Online).
26. Takizawa, T., and Robinson, J. M.: FluoroNanogold is a bifunctional immunoprobe for correlative fluorescence and electron microscopy J. Histochem. Cytochem., 48, 481-485 (2000).
    • Abstract (from JHC Online).
27. Takizawa, T., and Robinson, J. M.: FluoroNanogold as a probe for high resolution correlation between immunofluorescence and electron microscopy; Proc. 57^th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 476-477.
28. Takizawa, T.; Suzuki, K., and Robinson, J. M.: Correlative Microscopy Using FluoroNanogold on Ultrathin Cryosections: Proof of Principle; J. Histochem. Cytochem., 46, 1097-1102 (1998).
    • Abstract from JHC Online.

• Huang, S.; Deerinck, T. J.; Ellisman, M. H., and Spector, D. L.: In vivo analysis of the stability and transport of nuclear poly(A)+ RNA; J. Cell. Biol., 126, 877-899 (1994).
  • Abstract from the Journal of Cell Biology.

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In Situ Hybridization

1. Powell, R. D.; Joshi, V. N.; Halsey, C. M. R.; Hainfeld, J. F.; Hacker, G. W.; Hauser-Kronberger, C.; Muss, W. H., and Takvorian, P. M.: Combined Cy3 / Nanogold conjugates for immunocytochemistry and in situ hybridization; Proc. 57^th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 478-479.
   • See the complete paper

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Silver and Gold Enhancement

1. Keohane, E. M.; Orr, G. A.;Takvorian, P. M.;Cali, A.; Tanowitz, H. B.; Wittner, M., and Weiss, L. M.: Polar tube proteins of Microsporida of the family Encephalitozoonidae; J. Euk. Microbiol., 46, 1-5 (1999).
2. Powell, R. D., and Hainfeld, J. F.: Combined Fluorescent and Gold Probes for Microscopic and Morphological Investigations. In Gold and Silver Staining: Techniques in Molecular Morphology, (G. W. Hacker and J. Gu, Eds.), CRC Press, Boca Raton, FL; pp. 107-118 (2002).
3. Powell, R. D.; Halsey, C. M. R.; Spector, D. L.; Kaurin, S. L.; McCann, J.;, and Hainfeld, J. F. A covalent fluorescent-gold immunoprobe: "simultaneous" detection of a pre-mRNA splicing factor by light and electron microscopy. J. Histochem. Cytochem., 45, 947-956 (1997).
   • See the abstract from JHC Online.
4. Powell, R. D.; Halsey, C. M. R., and Hainfeld, J. F.: Combined fluorescent and gold immunoprobes: Reagents and methods for correlative light and electron microscopy. Microsc. Res. Tech., 42, 2-12 (1998).
5. Powell, R. D.; Joshi, V. N.; Halsey, C. M. R.; Hainfeld, J. F.; Hacker, G. W.; Hauser-Kronberger, C.; Muss, W. H., and Takvorian, P. M.: Combined Cy3 / Nanogold conjugates for immunocytochemistry and in situ hybridization; Proc. 57^th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 478-479.
   • See the complete paper
6. Robinson, J. M.; Takizawa, T.; Vandré, D. D., and Burry, R. W.: Ultrasmall immunogold particles: important probes for immunocytochemistry; Microsc. Res. Tech., 42, 13-23 (1998).
7. Robinson, J. M., and Vandré, D. D. Efficient immunocytochemical labeling of leukocyte microtubules with FluoroNanogold: An important tool for correlative microscopy. J. Histochem. Cytochem., 45, 631-642 (1997).
   • See the abstract from JHC Online.
8. Takizawa, T., and Robinson, J. M.: FluoroNanogold is a bifunctional immunoprobe for correlative fluorescence and electron microscopy J. Histochem. Cytochem., 48, 481-485 (2000).
   • Abstract (from JHC Online).

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