Fast. Simple. Sensitive. Reliable. Ni-NTA-Nanogold® binds directly to His-tags
No antibodies or special imaging equipment needed!
GoldiBlot™ uses our unique His-tag targeting gold nanoparticle probe, Ni-NTA-Nanogold® combined with fast, clean signal amplification using our autometalographic developer, GoldEnhance™.
The GoldiBlot™ process: Ni-NTA-Nanogold® binds to a polyhistidine (His) tag, then gold enhancement generates visible signal.
Features and Advantages
Economical: No expensive primary or secondary antibodies, or chemiluminescent or chromogenic substrates. Cost per blot is only about $22 (USD; based on 10 mL reagent volume per blot, or about one 7 x 8.4 cm blot).
Fast: Visualization is complete in about an hour. In contrast, protocols that use antibodies take about three hours to complete.
Universal: GoldiBlot™ detects His-tags regardless of position: C-terminal, N-terminal, or even internal. GoldiBlot™ can detect His-tagged fusion proteins that are sometimes undetectable with anti-His-tag antibodies.
Sensitive: the Ni2+- NTA interaction is as strong as antibody-antigen binding (binding constant ~1013 M-1), providing strong, specific binding at low concentrations and yielding clear, sensitive visualization.
Reliable: while the Ni2+- NTA interaction is as strong as antibody-antigen binding, it is a simple reaction that avoids the variability of anti-His tag antibodies.
Highly Specific: Ni2+ - NTA Nanogold® nanoparticles have similar binding capabilities to IMAC, and specifically detect polyhistidine tags without any requirement for specific adjacent groups.
Direct Visualization: No chemiluminescent substrates, X-ray film or CCD imagers.
Stable: all reagents are highly stable, with shelf life longer than three years.
Long-lived high contrast signal: dark purple-black staining is stable, long-lived and does not fade, making imaging and archiving straightforward.
GoldiBlot™ procedure and an example of the results, showing detection of His-tagged proteins from a variety of samples.
How GoldiBlot™ stacks up against the competition in cost and time.
Labeling and detection method:
Time to complete visualization:
Cost per blot (USD)*:
GoldiBlot™ His-tag Western Blot Kit
1 hour 15 minutes
$22.20
Chromogenic detection: peroxidase-labeled anti-His tag antibody and metal-enhanced DAB substrate
2 hours 50 minutes
$34.49
Chemiluminescent detection: peroxidase-labeled anti-His tag antibody and chemiluminescent HRP substrate
2 hours 50 minutes
$43.64
*Cost for blot using 10 mL reagent, about sufficient for one 7 x 8.4 cm blot. Volumes required and cost may be different for different sized or multiple blots.
Ni-NTA-Nanogold®: learn more
Our video introduction explains how Ni-NTA-Nanogold® works and how it compares with other methods for detecting His tags.
Ordering Information
Ordering: GoldiBlot™
Custom conjugation is also available, of Nanogold®, FluoroNanogold™, undecagold or colloidal gold to primary antibodies, peptides, small molecules, or other molecules.
International orders:Our regional distributors can provide expedited customs processing and delivery.
