References: GoldEnhance™

• Molecular TEM
• Tissue and immunohistochemistry: EM
• In Situ Hybridization

Molecular TEM

1. Scheibel, T.; Kowal, A. S.; Bloom, J. D., and Lindquist, S.L.: Bidirectional amyloid fiber growth for a yeast prion determinant. Current Biology, 11, 366-369 (2001).

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Tissue and Immunohistochemistry: EM

1. W. He, C. Kivork, S. MacHinani, M. Morphew, A. Gail, D. Tesar, N. Tiangco, J. McIntosh, P. Bjorkman, A freeze substitution fixation-based gold enlarging technique for EM studies of endocytosed nanogold-labeled molecules. J. Struct. Biol. 160(1), 103–113 (2007).
   • Contains a modified protocol for slower development with GoldEnhance™ EM (orginal formula). We now recommend GoldEnhance™ EM Plus, which is a slow developer with low background.
2. Ackerley, C. A.; Tilups, A.; Bear, C. E., and becker, L. E.: Correlative LM/TEM studies are essential in evaluating the effectiveness of liposome mediated delivery of the cystic fibrosis transmembrane regulator (CFTR) as a corrective therapy in a CFTR knockout mouse that develops lung disease; Proc. 57^th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 484-485.
3. Cheung, A. L.; Graf, A. H.; Hauser-Kronberger, C.; Dietze, O.; Tubbs, R. R., and Hacker, G. W.: Detection of human papillomavirus in cervical carcinoma: comparison or peroxidase, Nanogold, and catalyzed reporter deposition (CARD)-Nanogold in situ hybridization Mod. Pathol., 12, 689 (1999).
4. Grondin, G., and Beaudoin, A. R.: A New Pre-Embedding Immunogold Method that Permits to Obtain a Very High Signal with a Very Good Ultrastructure. Microsc. Microanal., 7, (Suppl. 2: Proceedings) (Proceedings of the Fifty-Ninth Annual Meeting, Microscopy Society of America); Bailey, G. W.; Price, R. L.; Voelkl, E., and Musselman, I. H., Eds.; Springer-Verlag, New York, NY, 2001, pp. 1044-1045.
5. Hainfeld, J. F.; Powell, R. D.; Stein, J. K.; Hacker, G. W.; Hauser-Kronberger, C.; Cheung, A. L. M., and Schofer, C.: Gold-based autometallography; Proc. 57^th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 486-487.
   • See the complete paper
6. Malecki, M.; Hsu, A.; Truong, L., and Sanchez, S: Molecular immunolabeling with recombinant single-chain variable fragment (scFv) antibodies designed with metal-binding domains; Proc. Natl. Acad. Sci. USA, 99, 213-218 (2002).
   • Abstract (courtesy of the National Academy of Sciences of the USA).
7. Powell, R. D.; Joshi, V. N.; Halsey, C. M. R.; Hainfeld, J. F.; Hacker, G. W.; Hauser-Kronberger, C.; Muss, W. H., and Takvorian, P. M.: Combined Cy3 / Nanogold conjugates for immunocytochemistry and in situ hybridization; Proc. 57^th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 478-479.
   • See the complete paper
8. Tubbs, R.; Pettay, J.; Skacel, M.; Powell, R.; Stoler, M.; Roche, P., and Hainfeld, J.: Gold-Facilitated in Situ Hybridization: A Bright-Field Autometallographic Alternative to Fluorescence in Situ Hybridization for Detection of HER-2/neu Gene Amplification. Am. J. Pathol., 160, 1589-1595 (2002).
   • Abstract (courtesy of the American Journal of Pathology)
9. Weipoltshammer, K.; Schéfer, C.; Almeder, M., and Wachtler, F.: Signal enhancement at the electron microscopic level using Nanogold and gold-based autometallography. Histochem. Cell Biol., 114, 489-495 (2000).
   • Abstract (courtesy of the Journal of Cell Biology).

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In Situ Hybridization

1. Graf, A. H.; Cheung, A. L.; Hauser-Kornberger, C.; Dandachi, N.; Tubbs, R. R.; Dietze, O., and Hacker, G. W.: Clinical relevance of HPV 16/18 testing methods in cervical squamous cell carcinoma. Appl. Immunohistochem. Molecul. Morphol., 8, 300-9 (2000).
2. Hainfeld, J. F.; Powell, R. D.; Stein, J. K.; Hacker, G. W.; Hauser-Kronberger, C.; Cheung, A. L. M., and Schofer, C.: Gold-based autometallography; Proc. 57^th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 486-487.
   • See the complete paper
3. Powell, R. D.; Joshi, V. N.; Halsey, C. M. R.; Hainfeld, J. F.; Hacker, G. W.; Hauser-Kronberger, C.; Muss, W. H., and Takvorian, P. M.: Combined Cy3 / Nanogold conjugates for immunocytochemistry and in situ hybridization; Proc. 57^th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 478-479.
   • See the complete paper
4. Weipoltshammer, K.; Schéfer, C.; Almeder, M., and Wachtler, F.: Signal enhancement at the electron microscopic level using Nanogold and gold-based autometallography. Histochem. Cell Biol., 114, 489-495 (2000).
   • Abstract (courtesy of the Journal of Cell Biology).

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