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1.4 nm Nanogold® Labeling Reagents
References by Application

 

 

 

General

  1. Bendayan, M.: Worth its weight in gold. Science, 291, 1363-5 (2001).
  2. Hainfeld, J. F.: Labeling with Nanogold and undecagold: techniques and results. Scanning Microsc. Suppl. (Proc. 14th Pfefferkorn Conf.); Malecki, M., and Roomans, G. M. (Eds.). Scanning Microscopy International, Chicago, IL, 10, 309-322 (1996).
  3. Hainfeld, J. F., and Powell, R. D.: New frontiers in gold labeling J. Histochem. Cytochem., 48, 471-480 (2000).
  4. Hainfeld, J. F., and Powell, R. D.: Nanogold Technology: New Frontiers in Gold Labeling. Cell Vision, 4, 408-432 (1997).
  5. Hainfeld, J. F., and Robinson, J. M.: New frontiers in gold labeling: Symposium overview J. Histochem. Cytochem., 48, 459-460 (2000).
  6. Hainfeld, J.F. Site specific cluster labels. Ultramicroscopy, 46, 135-144 (1992).
  7. Hainfeld, J.F. and Furuya, F.R. A 1.4nm Gold cluster covalently attached to antibodies improves immunolabeling, J. Histochem. Cytochem., 40, 177-184 (1992).
  8. Jahn, W.: Chemical aspects of the use of gold clusters in structural biology. J. Struct. Biol., 127, 106-112 (1999).
  9. Mahdi, F.; Madar, Z. S.; Figueroa, C. D., and Schmaier, A. H.: Factor XII interacts with the multiprotein assembly of urokinase plasminogen activator receptor, gC1qR, and cytokeratin 1 on endothelial cell membranes. Blood, 99, 3585-3596 (2002).
  10. Rayner, S. L., and Stephenson, F. A. Labelling and characterization of gamma-amminobutyric acidA receptor subunit-specific antibodies with monomaleimido-Nanogold. Biochem. Soc. Trans., 25, 546S (1997).
  11. Robinson, J. M.; Takizawa, T., and Vandré, D. D.: Applications of gold cluster compounds in immunocytochemistry and correlative microscopy: comparison with colloidal gold. J. Microsc., 199, 163-79 (2000).
  12. Robinson, J. M.; Takizawa, T., and Vandré: Enhanced immunolabeling efficiency using ultrasmall immunogold probes: Immunocytochemistry J. Histochem. Cytochem., 48, 487-492 (2000).
  13. Wall, J. S.: Visualizing "Greengold" clusters in the STEM. J. Struct. Biol., 127, 161-168 (1999).
  14. Yanase, K.; Smith, R. M.; Cizman, B.; Foster, M. H.; Peachey, L. D.; Jarrett, L., and Madaio, M. P.: A subgroup of Murine monoclonal anti-deoxyribonucleic acid antibodies traverse the cytoplasm and enter the nucleus in a time- and temperature- dependent manner; Laboratory Investigation, 71, 52-60 (1994).

 

Nanogold® Labeling Reagents: Catalog Information | Technical Help | Product Instructions

 

DNA and RNA Labeling

  1. Alivisatos, A. P., Johnsson, K. P., Peng, X., Wilson, T. E., Loweth, C. J., Bruchez, M. P., Jr., and Schultz, P. G.: Organization of 'Nanocrystal Molecules' using DNA. Nature, 382, 609 (1996).
  2. Dubertret, B., Calame, M., and Libchaber, A.; Nat. Biotechnol., 19, 365-370 (2001).
  3. Hamad-Schifferli, K.; Schwartz, J. J.; Santos, A. T.; Zhang, S., and Jacobson, J. M.: Remote electronic control of DNA hybridization through inductive coupling to an attached metal nanocrystal antenna. Nature, 415, 152-155 (2002).
  4. Malecki, M.: Energy filtering transmission electron microscopy of transfected DNA; In Proc 54th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, J. M. Corbett, R. V. W. Dimlich, J. R. Michael and N. J., Zaluzec (Eds.). San Francisco Press, San Francisco, CA, pp. 924-925 (1996).
  5. Malecki, M.: Preparation of plasmid DNA in transfection complexes for fluorescence and spectroscopic imaging. Scanning Microsc. Suppl. (Proc. 14th Pfefferkorn Conf.); Malecki, M., and Roomans, G. M. (Eds.). Scanning Microscopy International, Chicago, IL, 10, 1-16 (1996).
  6. Medalia, O.; Heim, M.; Guckenberger, R.; Sperling, R., and Sperling, J.: Gold-tagged RNA-A probe for macromolecular assemblies. J. Struct. Biol., 127, 113-119 (1999).

 

Nanogold® Labeling Reagents: Catalog Information | Technical Help | Product Instructions

 

DNA Nanowires and Quantum Dots

  1. Hainfeld, J. F.; ; Furuya, F. R.; Powell, R. D., and Liu, W. DNA Nanowires. Microsc. Microanal., 2001: 7(Suppl. 2: Proceedings / Proceedings of the Fifty-Ninth Annual Meeting, Microscopy Society of America), 1034-1035 (2001).

 

Nanogold® Labeling Reagents: Catalog Information | Technical Help | Product Instructions

 

Electron Microscopy: Tissue and Immunohistochemistry

EM: Pre-embedding Labeling

  1. Adams, I. R., and Kilmartin, J. V.: Localization of core spindle body (SPB) components during SPB duplication in Saccharomyces cerevisiae. J. Cell Biol., 145, 809-823 (1999).
  2. Baude, A.; Nusser, Z.; Molnar, E.; McIlhinney, R. A. J., and Somogyi, P.: High-resolution immunogold localization of AMPA type glutamate receptor subunits at synaptic and non-synaptic sites in rat hippocampus. Neuroscience, 69, 1031-1055 (1995).
    • Peter Somogyi: Medline search (results will be displayed in a new window).
  3. Baude, A., Nusser, A., Roberts, J.D.B., Mulvihill, E., McIlhinney, R.A.J., and Somogyi, P. The metabotropic glutamate receptor (mGluR1 a) is concentrated at perisynaptic membranes of neuronal subpopulations as detected by immunogold reaction. Neuron, 11, 771-787 (1993).
  4. Bergles, D. E.; Roberts, J. D. B.; Somogyi, P., and Jahr, C. E.: Glutamatergic synapses on oligodendrocyte precursor cells in the hippocampus. Nature, 405, 187-190 (2000).
  5. Bernard, V.; Levey, A. I., and Bloch, B.: Regulation of the subcellular distribution of m4 muscarinic acetylcholine receptors in striatal neurons in vivo by the cholinergic environment: evidence for regulation of cell surface receptors by endogenous and exogenous stimulation J. Neurosci., 19, 10237-10249 (1999).
  6. Bernard, V.; Somogyi, P., and Bolam, J. P.: Cellular, subcellular and subsynaptic distribution of AMPA-type glutamate receptor subunits in the neostriatum of the rat. J. Neuroscience, 17, 819-833 (1997).
  7. Burry, R.W.: Pre-embedding immunocytochemistry with silver-enhanced small gold particles, p. 217-230. In: Immunogold silver staining: Principles, methods and applications, (M. A. Hayat, Ed.), CRC Press, Boca Raton, FL; p. 217-230 (1995).
  8. Burry, R.W., Vandré, D. D., and Hayes, D. M.: Silver enhancement of gold antibody probes in pre-embedding electron microscopic immunocytochemistry. J. Histochem. Cytochem., 40, 1849-1856 (1992).
  9. D'Este, L.; Kulaksiz, H.; Rausch, U.; Vaccaro, R.; Wenger, T.; Tokunaga, Y.; Renda, T. G.; Cetin, Y.: Expression of guanylin in "pars tuberalis-specific cells" and gonadotrophs of rat adenohypophysis Proc. Natl. Acad. Sci. USA, 97, 1131-1136 (2000).
  10. Du, J.; Tao-Cheng, J.-H.; Zerfas, P., and McBain, C. J. The K+ channel, Kv2.1, is apposed to astrocytic processes and is associated with inhibitory postsynaptic membranes in hippocampal and cortical principal neurons and inhibitory interneurons. Neuroscience, 84, 37-48 (1998).
  11. Fagotto, F.; Jho, E.-H.; Zeng, L.; Kurth, T.; Joos, T.; Kaufmann, C., and Constantini, F.: Domains of axin involved in protein-protein interactions, Wnt pathway inhibition, and intracellular localization. J. Cell Biol., 145, 741-756 (1999).
  12. Feng, D.; Nagy, J. A.; Brekken, R. A.; Pettersson, A.; Manseau, E. J.; Pyne, L.; Mulligan, R.; Thorpe, P. E.; Dvorak, H. F., and Dvorak, A. M.: Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF-expressing tumors and adenoviral vectors J. Histochem. Cytochem., 48, 545-555 (2000).
  13. Gilerovitch, H.G., Bishop, G.A., King, J.S., and Burry, R.W. The use of electron microscopic immunocytochemistry with silver-enhanced 1.4nm gold particles to localize GAD in the cerebellar nuclei. J. Histochem. Cytochem., 43, 337-43 (1995).
  14. Gilerovitch, H.G., Bishop, G.A., King, J.S., and Burry, R.W. Demonstration of GAD in Purkinje-cell terminals with silver enhanced gold immunocytochemistry. In G. Bailey and C.L. Rieder (Eds.), Proc. 51st Ann. Mtg. Micros. Soc. Amer., San Francisco Press, pp. 288-289 (1993).
  15. Grondin, G., and Beaudoin, A. R.: A New Pre-Embedding Immunogold Method that Permits to Obtain a Very High Signal with a Very Good Ultrastructure. Microsc. Microanal., 7, (Suppl. 2: Proceedings) (Proceedings of the Fifty-Ninth Annual Meeting, Microscopy Society of America); Bailey, G. W.; Price, R. L.; Voelkl, E., and Musselman, I. H., Eds.; Springer-Verlag, New York, NY, 2001, pp. 1044-1045.
  16. Gutekunst, C.-A.; Li, S.-H.; Yi, H.;Mulroy, J. S.; Kuemmerle, S.; Jones, R.; Rye, D.; Ferrante, R. J.; Hersch, S. M., and Li, X.-J.: Nuclear and Neuropil Aggregates in Huntington's Disease: Relationship to Neuropathology. J. Neuroscience, 19, 2522-2534 (1999).
  17. Hanson, J. E., and Smith, Y.: Group I metabotropic glutamate receptors at GABAergic synapses in monkeys; J. Neurosci., 19, 6488-6496 (1999).
  18. Hearne, C. E.; Johnson, D. L., and Van Campen, H.: Immunogold-Silver Staining (IGSS) of (NCP and CP) BVDV-infected subcellular fraction bands; In Proc 55th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, R. V. W. Dimlich, K. B. Alexander, J. J. McCarthy and T. P. Pretlow (Eds.). Springer-Verlag, New York, NY, pp. 155-156 (1997).
  19. Hearne, C. E., and Van Campen, H.: Immunogold-Silver Staining (IGSS) of agarose embedded (NCP) BVDV-infected cell suspensions; In Proc 54th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, J. M. Corbett, R. V. W. Dimlich, J. R. Michael and N. J., Zaluzec (Eds.). San Francisco Press, San Francisco, CA, pp. 902-903 (1996).
  20. Humbel, B. M.; De Jong, M. D. M.; Müller, W. H., and Verkleij, A. J.: Pre-embedding immunolabeling for electron microscopy: An evaluation of permeabilization methods and markers. Microsc. Res. Tech., 42, 43-58 (1998).
  21. Ikeda, Y.; Martone, M.; Gu, Y.; Hoshijima, M.; Thor, A.; Oh, S. S.; Peterson, K. L., and Ross, J., Jr.: Altered membrane proteins and permeability correlate with cardiac dysfunction in cardiomyopathic hamsters Am. J. Physiol. Heart Circ. Physiol., 278, H1362-H1370 (2000).
  22. Li, H.; Ohishi, H.; Kinoshita, A.; Shigemoto, R.; Nomura, S., and Mizuno, N.: Localization of a metabotropic glutamate receptor, mGluR7, in axon terminals of presumed nociceptive, primary afferent fibers in the superficial layers of the spinal dorsal horn: an electron microscope study in the rat. Neuroscience lett., 223, 153-156 (1997).
  23. Li, S.-H.; Cheng, A. L.; Li, H., and Li, X.-J.: Cellular effects and altered gene expression in PC12 cells stably expressing mutant huntingtin. J. Neuroscience, 19, 5159-5172 (1999).
  24. Lin, M., Sistina, Y., and Rodger, J. C.: Electron-microscopic localisation of thiol and disulphide groups by direct monomaleimido-Nanogold labeling in the spermatozoa of a marsupial, the tammar wallaby (Macropus eugenii); Cell Tisue Res., 282, 291-296 (1995).
  25. Lujan, R.; Nusser, Z.; Roberts, J. D. B.; Shigemoto R.; Ohishi, H., and Somogyi, P.: Differential plasma membrane distribution of metabotropic glutamate receptors mGluR1-alpha, mGluR1 and mGluR5, relative to neurotransmitter release sites. J. Chem. Neuroanat., 13, 219-241 (1997).
  26. Lujan, R.; Nusser, Z.; Roberts, J. D. B.; Shigemoto R., and Somogyi, P.: Perisynaptic location of metabotropic glutamate receptors mGluR1 and mGluR5 on dendrites and dendritic spines in the rat hippocampus. Eur. J. Neuroscience, 8, 1488-1500 (1996).
  27. Matsuzaki, T.; Suzuki, T.; Koyama, H.; Tanaka, S., and Takata, K.: Water channel protein AQP3 is present in epithelia exposed to the environment of possible water loss. J. Histochem. Cytochem., 47, 1275-1286 (1999).
  28. Mizoguchi, A.; Yano, Y.; Hamaguchi, H.; Yanagida, H.; Ide, C.; Zahraoui, A.; Shirataki, H.; Sasaki, T., and Takai, Y.: Localization of Rabphilin-3A on the synaptic vesicle. Biochem. Biophys. Res. Commun, 202, 1235-1243 (1999).
  29. Nixon, G. F., Mignery, G. A., and Somlyo, A. V.: Immunogold localization of inositol 1,4,5-trisphosphate receptors and characterization of ultrastructural features of the sarcoplasmic reticulum in phasic and tonic smooth muscle; J. Muscle Res. Cell Mot., 15, 682-700 (1994).
  30. Nusser, Z.; Hajos, N.; Somogyi, P., and Mody, I.: Increased number of synaptic GABAA receptors underlies potentiation at hippocampal inhibitory synapses; Nature, 395, 172-177 (1998).
  31. Nusser, Z., Sieghart, W., and Somogyi, P.: Segregation of different GABAA receptors to synaptic and extrasynaptic membranes of cerebellar granule cells. J. Neuroscience, 18, 1693-1703 (1998).
  32. Nusser, Z., and Somogyi, P.: Compartmentalised distribution of GABAA and glutamate receptors in relation to transmitter release sites on the surface of cerebellar neurones. Prog. Brain Research, (C. I. De Zeeuw, P. Strata and J. Voogd, Eds.), Elsevier, Oxford, UK.; 114, 1488-1500 (1997).
  33. Nusser, Z.; Sieghart, W.; Stephenson, F. A., and Somogyi, P.: The alpha-6 subunit of the GABAA receptor is concentrated in both inhibitory and excitatory synapses on cerebellar granule cells. J. Neuroscience, 16, 103-114 (1996).
  34. Nusser, Z.; Roberts, J. D. B.; Baude, A.; Richards, J. G., and Somogyi, P. Relative densities of synaptic and extrasynaptic GABA-A receptors on cerebellar granule cells as determined by a quantitative immunogold method. J. Neuroscience; 15, 2948-2960 (1995).
  35. Nusser, Z.; Roberts, J. D. B.; Baude, A.; Richards, J. G.; Sieghart, W., and Somogyi. P. Immunocytochemical localization of the alpha-1 and beta-2/3 subunits of the GABA-A receptor in relation to specific GABAergic synapses in the dentate gyrus. Eur. J. Neurosci., 7, 630-646 (1995).
  36. Prescianotto-Baschong, C., and Riezman, H.: Morphology of the yeast endocytic pathway. Mol. Cell Biol., 9, 173-189 (1998).
  37. Robinett, C. C., Straight, A., Li, G., Willhelm, C., Sudlow, G., Murray, A., and Belmont, A. S.: In vivo localization of DNA sequences and visualization of large-scale chromatin organization using lac operator/repressor recognition. J. Cell Biol., 135, 1685-1700 (1996).
  38. Sawada, H., and Esaki, M.: A practical technique to postfix Nanogold-immunolabeled specimens with osmium and to embed them in Epon for electron microscopy J. Histochem. Cytochem., 48, 493-498 (2000).
  39. Sawada, H., Sugawara, I., Kitami, A., and Hayashi, M. Vitronectin in the cytoplasm of Leydig cells in the rat testis. Biol. Reprod., 54, 29-35 (1996).
  40. Sawada, H., and Esaki, H: Use of Nanogold followed by silver enhancement and gold toning for preembedding immunolocalization. J. Elect. Micro., 43, 361-66 (1994).
  41. Somogyi, P.; Tamas, G.; Lujan, R., and Buhl, E. H.: Salient features of synaptic organization in the cerebral cortex. Brain Res., Brain Res. Rev., 26 113-135 (1999).
  42. Suzuki, E., and Hirosawa, K. Immunolocalization of a Drosophila phosphatidylinositol transfer protein (rdgB) in normal and rdgA mutant photoreceptor cells with special reference to the subrhabdomeric cisternae. J. Electron Microsc, 43, 183-9 (1994).
  43. Soussan, L.; Burakov, M.; Daniels, M. P.; Toister-Achituv, M.; Porat, A.; Yarden, Y., and Elazar, Z.: ERG30, a VAP-33-related protein, functions in protein transport mediated by COPI vesicles. J. Cell Biol., 146, 301-311 (1999).
  44. Suzuki, Y.; Itakura, M.; Kashiwagi, M.; Nakamura, N.; Matsuki, T.; Sakuta, H.; Naito, N.; Takano, K.; Fujita, T., and Hirose, S.: Identification by differential display of a hypertonicity-inducible inward rectifier potassium channel highly expressed in chloride cells. J. Biol. Chem, 274, 11376-11382 (1999).
  45. Tanner, V. A., Ploug, T., and Tao-Cheng, J.-H. Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM Immunocytochemistry for cell cultures. J. Histochem. Cytochem., 44, 1481-1488 (1996).
  46. Tao-Cheng, J.-H. and Tanner, V.A. A modified method of pre-embedding EM immunocytochemistry which improves specificity and simplifies the process for in vitro cells. In G. Bailey and A.J. Garratt-Reed (Eds.), Proc. 52nd Ann. Mtg. Micros. Soc. Amer.; (pp. 306-307). San Francisco Press (1994).
  47. Thompson, W. F., Beven, A. F., Wells, B., and Shaw, P. J. Sites of rDNA transcription genes are widely dispersed through the nucleolus in Pisum sativum and can comprise single genes. Plant J., 12, 571-581 (1997).
  48. Tolstonog, G. V.; Sabasch, M., and Traub, P.: Cytoplasmic Intermediate Filaments Are Stably Associated with Nuclear Matrices and Potentially Modulate Their DNA-Binding Function. DNA Cell Biol., 21, 213-39 (2002).
  49. Vandré, D.D. and Burry, R.W. Immunoelectron microscopic localization of phosphoproteins associated with the mitotic spindle. J. Histochem.Cytochem., 40, 1837-1847 (1992).
  50. Yanase, K.; Smith, R. M.; Cizman, B.; Foster, M. H.; Peachey, L. D.; Jarrett, L., and Madaio, M. P.: A subgroup of Murine monoclonal anti-deoxyribonucleic acid antibodies traverse the cytoplasm and enter the nucleus in a time- and temperature- dependent manner; Laboratory Investigation, 71, 52-60 (1994).
  51. Yazama, F., Esaki, M., and Sawada, H.: Immunocytochemistry of Extracellular Matric Components in the Rat Seminiferous Tubule: Electron Microscopic Localization with Improved Methodology. Anat. Rec., 248, 51-62 (1997).
  52. Yoshida, M.;Wakatsuki, Y.; Kobayashi, Y.; Itoh, T.; Murakami, K.; Mizoguchi, A.; Usui, T.; Chiba, T., and Kita, T.; Cloning and Characterization of a Novel Membrane-Associated Antigenic Protein of Helicobacter pylori. Infection and Immunity, 67, 286-293 (1999).
  53. Yoshimori, T.; Yamagata, F.; Yamamoto, A.; Mizushima, N.; Kabeya, Y.; Nara, A.; Miwako, I.; Ohashi, M.; Ohsumi, M., and Ohsumi, Y.: The Mouse SKD1,a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells Mol. Biol. Cell, 11, 747-763 (2000).
  54. Zirwes, R. F.; Eilbracht, J.; Kneissel, S., and Schmidt-Zachmann, M. S.: A novel helicase-type protein in the Nucleolus: protein NOH61 Mol. Biol. Cell, 11, 1153-1167 (2000).

 

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EM: Post-embedding Labeling

  1. Baude, A.; Nusser, Z.; Molnar, E.; McIlhinney, R. A. J., and Somogyi, P.: High-resolution immunogold localization of AMPA type glutamate receptor subunits at synaptic and non-synaptic sites in rat hippocampus. Neuroscience, 69, 1031-1055 (1995).
    • Peter Somogyi: Medline search (results will be displayed in a new window).
  2. Baude, A., Nusser, A., Roberts, J.D.B., Mulvihill, E., McIlhinney, R.A.J., and Somogyi, P. The metabotropic glutamate receptor (mGluR1 a) is concentrated at perisynaptic membranes of neuronal subpopulations as detected by immunogold reaction. Neuron, 11, 771-787 (1993).
  3. Bernard, V.; Somogyi, P., and Bolam, J. P.: Cellular, subcellular and subsynaptic distribution of AMPA-type glutamate receptor subunits in the neostriatum of the rat. J. Neuroscience, 17, 819-833 (1997).
  4. Gimenez-Bonafe, P.; Ribes, E.; Sautière, P.; Gonzalez, A.; Kasinsky, H.; Kouach, M.; Sautière, P.-E.; Ausio, J., and Chiva, M.: Chromatin condensation, cysteine-rich protamine, and establishment of disulphide interprotamine bonds during spermiogenesis of Eledone cirrhosa (Cephalopoda). Eur. J. Cell Biol., 81, 341-349 (2002).
  5. Gardiol, A., Racca, C., and Triller, A.: Dendritic and Postsynaptic Protein Synthetic Machinery. J. Neuroscience, 19, 168-179 (1999).
  6. Halasy, K.; Buhl, E. H.; Lörinczi, Z.; Tamás, G., and Somogyi, P: Synaptic target selectivity and input of GABAergic basket and bistratified interneurons in the CA1 area of the rat hippocampus. Hippocampus, 6, 306-329 (1996).
  7. Krenács, T., and Krenács, L.: Immunogold-silver staining (IGSS) for single and multiple antigen detection in archived tissues following antigen retrieval. Cell Vision, 4, 387-393 (1997).
  8. Krenács, T. and Dux, L. Silver-enhanced immunogold labeling of calcium-ATPase in sarcoplasmic reticulum of skeletal muscle. J. Histochem. Cytochem., 42, 967-968 (1994).
  9. Krenács, T., and Krenács, L.: Comparison of embedding media for immunogold-silver staining. In Immunogold-Silver Staining: Principles, Methods and Applications," M. A. Hayat (Ed.); CRC Press, Boca Raton, FL, 1995, pp. 57-70.
  10. Lipschitz, D. L., and Michel, W. C.: Physiological Evidence for the discrimination of L-arginine from structural analogues by the zebrafish olfactory system J. Neurophysiol., 82, 3160-3167 (1999).
  11. Lujan, R.; Nusser, Z.; Roberts, J. D. B.; Shigemoto R.; Ohishi, H., and Somogyi, P.: Differential plasma membrane distribution of metabotropic glutamate receptors mGluR1-alpha, mGluR1 and mGluR5, relative to neurotransmitter release sites. J. Chem. Neuroanat., 13, 219-241 (1997).
  12. Lujan, R.; Nusser, Z.; Roberts, J. D. B.; Shigemoto R., and Somogyi, P.: Perisynaptic location of metabotropic glutamate receptors mGluR1 and mGluR5 on dendrites and dendritic spines in the rat hippocampus. Eur. J. Neuroscience, 8, 1488-1500 (1996).
  13. Matsubara, A., Laake, J. H., Davanger, S., Usami, S.-I., and Otterson, O. P.; Organization of AMPA receptor subunits at a glustamate synapse: A quantitative immunogold analysis of hair cell synapses in the rat organ of Corti. J. Neuroscience, 16, 4457-4467 (1996).
  14. Nusser, Z.; Cull-Candy, S., and Farrant, M.; Differences in synaptic GABAA receptor number underlie variation in GABA mini amplitude; Neuron, 19, 697-709 (1997).
  15. Nusser, Z.; Hajos, N.; Somogyi, P., and Mody, I.: Increased number of synaptic GABAA receptors underlies potentiation at hippocampal inhibitory synapses; Nature, 395, 172-177 (1998).
  16. Nusser, Z., and Somogyi, P.: Compartmentalised distribution of GABAA and glutamate receptors in relation to transmitter release sites on the surface of cerebellar neurones. Prog. Brain Research, (C. I. De Zeeuw, P. Strata and J. Voogd, Eds.), Elsevier, Oxford, UK.; 114, 1488-1500 (1997).
  17. Nusser, Z.; Sieghart, W.; Benke, D.; Fritschy, J.-M., and Somogyi, P.: Differential synaptic localization of two major gamma-aminobutyric acid type A receptor alpha subunits on hippocampal pyramidal cells. Proc. Natl. Acad. Sci. USA, 93, 11939-11944 (1996).
  18. Nusser, Z.; Sieghart, W.; Stephenson, F. A., and Somogyi, P.: The alpha-6 subunit of the GABAA receptor is concentrated in both inhibitory and excitatory synapses on cerebellar granule cells. J. Neuroscience, 16, 103-114 (1996).
  19. Nusser, Z.; Mulvihill, E.; Streit, P., and Somogyi, P.: Subsynaptic segregation of metabotropic and ionotropic glutamate receptors as revealed by immunogold localization. Neuroscience, 61, 421-427 (1994).
  20. Nusser, Z.; Roberts, J. D. B.; Baude, A.; Richards, J. G., and Somogyi, P. Relative densities of synaptic and extrasynaptic GABA-A receptors on cerebellar granule cells as determined by a quantitative immunogold method. J. Neuroscience; 15, 2948-2960 (1995).
  21. Nusser, Z.; Roberts, J. D. B.; Baude, A.; Richards, J. G.; Sieghart, W., and Somogyi. P. Immunocytochemical localization of the alpha-1 and beta-2/3 subunits of the GABA-A receptor in relation to specific GABAergic synapses in the dentate gyrus. Eur. J. Neurosci., 7, 630-646 (1995).
  22. Salas, P. J. I.: Insoluble gamma-tubulin-containing structures are anchored to the apical network of intermediate filaments in polarized CACO-2 epithelial cells. J. Cell Biol., 146, 645-657 (1999).
  23. Qualmann, B.; Kessels, M. M.; Thole, H. H., and Sierralta, W. D.; A hormone pulse induces transient changes in the subcellular distribution and leads to a lysosomal accumulation of the estradiol receptor alpha in target tissues. Eur. J. Cell Biol., 79, 383-93 (2000).
  24. Shigemoto, R., Kulik, A., Roberts, J. D. B., Ohishi, H., Nusser, Z., Kaneko, T., and Somogyi, P.; Target-cell-specific concentration of a metabotropic glutamate receptor in the presynaptic active zone. Nature, 381, 523-525 (1996).
  25. Somogyi, P.; Tamas, G.; Lujan, R., and Buhl, E. H.: Salient features of synaptic organization in the cerebral cortex. Brain Res., Brain Res. Rev., 26 113-135 (1999).
  26. Weipoltshammer, K.; Schéfer, C.; Almeder, M., and Wachtler, F.: Signal enhancement at the electron microscopic level using Nanogold and gold-based autometallography. Histochem. Cell Biol., 114, 489-495 (2000).

 

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EM: Ultrathin Cryosections

  1. Ackerly, C. A., Becker, L. E., Tilups, A., Rutlka, J. T., and Mancuso, J. F.: CCD Cameras facilitate the imaging of small gold particles in immunogold-labelled ultrathin cryosections. In Proc 54th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, J. M. Corbett, R. V. W. Dimlich, J. R. Michael and N. J., Zaluzec (Eds.). San Francisco Press, San Francisco, CA, pp. 904-905 (1996).
  2. Baude, A., Nusser, A., Roberts, J.D.B., Mulvihill, E., McIlhinney, R.A.J., and Somogyi, P. The metabotropic glutamate receptor (mGluR1 a) is concentrated at perisynaptic membranes of neuronal subpopulations as detected by immunogold reaction. Neuron, 11, 771-787 (1993).
  3. Robinson, J. M.; Takizawa, T.; Vandré, D. D., and Burry, R. W.: Ultrasmall immunogold particles: important probes for immunocytochemistry; Microsc. Res. Tech., 42, 13-23 (1998).
  4. Robinson, J. M., and Takizawa, T.: Novel Labeling methods for EM analysis of ultrathin cryosections. In Proc 54th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, J. M. Corbett, R. V. W. Dimlich, J. R. Michael and N. J., Zaluzec (Eds.). San Francisco Press, San Francisco, CA, pp. 894-895 (1996).
  5. Takizawa, T. and Robinson, J.M. Use of 1.4-nm immunogold particles for immunocytochemistry on ultra-thin cryo- sections. J. Histochem. Cytochem., 42, 1615-1623 (1994).

 

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EM: Electron Crystallography

  1. Wille, H.; Michelitsch, M. D.; Guenebaut, V.; Supattapone, S.; Serban, A.; Cohen, F. E.; Agard, D. A, and Prusiner, S. B.: Structural studies of the scrapie prion protein by electron crystallography. Proc. Natl. Acad. Sci. USA, 99, 3563-8 (2002).

 

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Enhancement

Gold Enhancement

  1. Ackerley, C. A.; Tilups, A.; Bear, C. E., and becker, L. E.: Correlative LM/TEM studies are essential in evaluating the effectiveness of liposome mediated delivery of the cystic fibrosis transmembrane regulator (CFTR) as a corrective therapy in a CFTR knockout mouse that develops lung disease; Proc. 57th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 484-485.
  2. Cheung, A. L.; Graf, A. H.; Hauser-Kronberger, C.; Dietze, O.; Tubbs, R. R., and Hacker, G. W.: Detection of human papillomavirus in cervical carcinoma: comparison or peroxidase, Nanogold, and catalyzed reporter deposition (CARD)-Nanogold in situ hybridization Mod. Pathol., 12, 689 (1999).
  3. Graf, A. H.; Cheung, A. L.; Hauser-Kornberger, C.; Dandachi, N.; Tubbs, R. R.; Dietze, O., and Hacker, G. W.: Clinical relevance of HPV 16/18 testing methods in cervical squamous cell carcinoma. Appl. Immunohistochem. Molecul. Morphol., 8, 300-9 (2000).
  4. Hainfeld, J. F.; Powell, R. D.; Stein, J. K.; Hacker, G. W.; Hauser-Kronberger, C.; Cheung, A. L. M., and Schofer, C.: Gold-based autometallography; Proc. 57th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 486-487.
  5. Powell, R. D.; Joshi, V. N.; Halsey, C. M. R.; Hainfeld, J. F.; Hacker, G. W.; Hauser-Kronberger, C.; Muss, W. H., and Takvorian, P. M.: Combined Cy3 / Nanogold conjugates for immunocytochemistry and in situ hybridization; Proc. 57th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 478-479.
  6. Scheibel, T.; Kowal, A. S.; Bloom, J. D., and Lindquist, S.L.: Bidirectional amyloid fiber growth for a yeast prion determinant. Current Biology, 11, 366-369 (2001).
  7. Tubbs, R.; Pettay, J.; Skacel, M.; Powell, R.; Stoler, M.; Roche, P., and Hainfeld, J.: Gold-Facilitated in Situ Hybridization: A Bright-Field Autometallographic Alternative to Fluorescence in Situ Hybridization for Detection of HER-2/neu Gene Amplification. Am. J. Pathol., 160, 1589-1595 (2002).
  8. Weipoltshammer, K.; Schéfer, C.; Almeder, M., and Wachtler, F.: Signal enhancement at the electron microscopic level using Nanogold and gold-based autometallography. Histochem. Cell Biol., 114, 489-495 (2000).

 

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Silver Enhancement

  1. Baude, A.; Nusser, Z.; Molnar, E.; McIlhinney, R. A. J., and Somogyi, P.: High-resolution immunogold localization of AMPA type glutamate receptor subunits at synaptic and non-synaptic sites in rat hippocampus. Neuroscience, 69, 1031-1055 (1995).
  2. Bergles, D. E.; Roberts, J. D. B.; Somogyi, P., and Jahr, C. E.: Glutamatergic synapses on oligodendrocyte precursor cells in the hippocampus. Nature, 405, 187-190 (2000).
  3. Bernard, V.; Levey, A. I., and Bloch, B.: Regulation of the subcellular distribution of m4 muscarinic acetylcholine receptors in striatal neurons in vivo by the cholinergic environment: evidence for regulation of cell surface receptors by endogenous and exogenous stimulation J. Neurosci., 19, 10237-10249 (1999).
  4. Bernard, V.; Somogyi, P., and Bolam, J. P.: Cellular, subcellular and subsynaptic distribution of AMPA-type glutamate receptor subunits in the neostriatum of the rat. J. Neuroscience, 17, 819-833 (1997).
  5. Burry, R.W.: Pre-embedding immunocytochemistry with silver-enhanced small gold particles, p. 217-230. In: Immunogold silver staining: Principles, methods and applications, (M. A. Hayat, Ed.), CRC Press, Boca Raton, FL; p. 217-230 (1995).
  6. Burry, R.W., Vandré, D. D., and Hayes, D. M.: Silver enhancement of gold antibody probes in pre-embedding electron microscopic immunocytochemistry. J. Histochem. Cytochem., 40, 1849-1856 (1992).
  7. Cheung, A. L.; Graf, A. H.; Hauser-Kronberger, C.; Dietze, O.; Tubbs, R. R., and Hacker, G. W.: Detection of human papillomavirus in cervical carcinoma: comparison or peroxidase, Nanogold, and catalyzed reporter deposition (CARD)-Nanogold in situ hybridization Mod. Pathol., 12, 689 (1999).
  8. D'Este, L.; Kulaksiz, H.; Rausch, U.; Vaccaro, R.; Wenger, T.; Tokunaga, Y.; Renda, T. G.; Cetin, Y.: Expression of guanylin in "pars tuberalis-specific cells" and gonadotrophs of rat adenohypophysis Proc. Natl. Acad. Sci. USA, 97, 1131-1136 (2000).
  9. Du, J.; Tao-Cheng, J.-H.; Zerfas, P., and McBain, C. J. The K+ channel, Kv2.1, is apposed to astrocytic processes and is associated with inhibitory postsynaptic membranes in hippocampal and cortical principal neurons and inhibitory interneurons. Neuroscience, 84, 37-48 (1998).
  10. Fagotto, F.; Jho, E.-H.; Zeng, L.; Kurth, T.; Joos, T.; Kaufmann, C., and Constantini, F.: Domains of axin involved in protein-protein interactions, Wnt pathway inhibition, and intracellular localization. J. Cell Biol., 145, 741-756 (1999).
  11. Feng, D.; Nagy, J. A.; Brekken, R. A.; Pettersson, A.; Manseau, E. J.; Pyne, L.; Mulligan, R.; Thorpe, P. E.; Dvorak, H. F., and Dvorak, A. M.: Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF-expressing tumors and adenoviral vectors J. Histochem. Cytochem., 48, 545-555 (2000).
  12. Gardiol, A., Racca, C., and Triller, A.: Dendritic and Postsynaptic Protein Synthetic Machinery. J. Neuroscience, 19, 168-179 (1999).
  13. Gutekunst, C.-A.; Li, S.-H.; Yi, H.;Mulroy, J. S.; Kuemmerle, S.; Jones, R.; Rye, D.; Ferrante, R. J.; Hersch, S. M., and Li, X.-J.: Nuclear and Neuropil Aggregates in Huntington's Disease: Relationship to Neuropathology. J. Neuroscience, 19, 2522-2534 (1999).
  14. Hainfeld, J. F., and Furuya, F. R.: Silver Enhancement of Nanogold and Undecagold. In Immunogold-Silver Staining: Principles, Methods and Applications," M. A. Hayat (Ed.); CRC Press, Boca Raton, FL, 1995, pp. 71-96.
  15. Halasy, K.; Buhl, E. H.; Lörinczi, Z.; Tamás, G., and Somogyi, P: Synaptic target selectivity and input of GABAergic basket and bistratified interneurons in the CA1 area of the rat hippocampus. Hippocampus, 6, 306-329 (1996).
  16. Hanson, J. E., and Smith, Y.: Group I metabotropic glutamate receptors at GABAergic synapses in monkeys; J. Neurosci., 19, 6488-6496 (1999).
  17. Ikeda, Y.; Martone, M.; Gu, Y.; Hoshijima, M.; Thor, A.; Oh, S. S.; Peterson, K. L., and Ross, J., Jr.: Altered membrane proteins and permeability correlate with cardiac dysfunction in cardiomyopathic hamsters Am. J. Physiol. Heart Circ. Physiol., 278, H1362-H1370 (2000).
  18. Kohler, A.; Lauritzen, B., and Van Noorden, J. F.: Signal amplification in immunohistochemistry at the light microscopic level using biotinylated tyramide and Nanogold-silver staining J. Histochem. Cytochem., 48, 933-941 (2000).
  19. Lipschitz, D. L., and Michel, W. C.: Physiological Evidence for the discrimination of L-arginine from structural analogues by the zebrafish olfactory system J. Neurophysiol., 82, 3160-3167 (1999).
  20. Lujan, R.; Nusser, Z.; Roberts, J. D. B.; Shigemoto R.; Ohishi, H., and Somogyi, P.: Differential plasma membrane distribution of metabotropic glutamate receptors mGluR1-alpha, mGluR1 and mGluR5, relative to neurotransmitter release sites. J. Chem. Neuroanat., 13, 219-241 (1997).
  21. Lujan, R.; Nusser, Z.; Roberts, J. D. B.; Shigemoto R., and Somogyi, P.: Perisynaptic location of metabotropic glutamate receptors mGluR1 and mGluR5 on dendrites and dendritic spines in the rat hippocampus. Eur. J. Neuroscience, 8, 1488-1500 (1996).
  22. Mahdi, F.; Madar, Z. S.; Figueroa, C. D., and Schmaier, A. H.: Factor XII interacts with the multiprotein assembly of urokinase plasminogen activator receptor, gC1qR, and cytokeratin 1 on endothelial cell membranes. Blood, 99, 3585-3596 (2002).
  23. Malecki, M.; Hsu, A.; Truong, L., and Sanchez, S: Molecular immunolabeling with recombinant single-chain variable fragment (scFv) antibodies designed with metal-binding domains; Proc. Natl. Acad. Sci. USA, 99, 213-218 (2002).
  24. Matsubara, A., Laake, J. H., Davanger, S., Usami, S.-I., and Otterson, O. P.; Organization of AMPA receptor subunits at a glustamate synapse: A quantitative immunogold analysis of hair cell synapses in the rat organ of Corti. J. Neuroscience, 16, 4457-4467 (1996).
  25. Nusser, Z.; Cull-Candy, S., and Farrant, M.; Differences in synaptic GABAA receptor number underlie variation in GABA mini amplitude; Neuron, 19, 697-709 (1997).
  26. Nusser, Z., Sieghart, W., and Somogyi, P.: Segregation of different GABAA receptors to synaptic and extrasynaptic membranes of cerebellar granule cells. J. Neuroscience, 18, 1693-1703 (1998).
  27. Nusser, Z., and Somogyi, P.: Compartmentalised distribution of GABAA and glutamate receptors in relation to transmitter release sites on the surface of cerebellar neurones. Prog. Brain Research, (C. I. De Zeeuw, P. Strata and J. Voogd, Eds.), Elsevier, Oxford, UK.; 114, 1488-1500 (1997).
  28. Nusser, Z.; Sieghart, W.; Benke, D.; Fritschy, J.-M., and Somogyi, P.: Differential synaptic localization of two major gamma-aminobutyric acid type A receptor alpha subunits on hippocampal pyramidal cells. Proc. Natl. Acad. Sci. USA, 93, 11939-11944 (1996).
  29. Nusser, Z.; Sieghart, W.; Stephenson, F. A., and Somogyi, P.: The alpha-6 subunit of the GABAA receptor is concentrated in both inhibitory and excitatory synapses on cerebellar granule cells. J. Neuroscience, 16, 103-114 (1996).
  30. Nusser, Z.; Mulvihill, E.; Streit, P., and Somogyi, P.: Subsynaptic segregation of metabotropic and ionotropic glutamate receptors as revealed by immunogold localization. Neuroscience, 61, 421-427 (1994).
  31. Prescianotto-Baschong, C., and Riezman, H.: Morphology of the yeast endocytic pathway. Mol. Cell Biol., 9, 173-189 (1998).
  32. Pohl, K., and Stierhof, Y.-D.: Action of gold chloride ("Gold toning") on silver-enhanced 1 nm gold markers. Microsc. Res. Tech., 42, 66-79 (1998).
  33. Punnonen, E.-L., Fages, C., Wartiovaara, J., and Rauvala, H.: Ultrststructural Localization of beta-Actin and Amphoterin mRNA in Cultured Cells: Application of tyramide signal amplification and comparison of detection methods; J. Histochem. Cytochem., 47, 99 (1999).
  34. Qualmann, B.; Kessels, M. M.; Thole, H. H., and Sierralta, W. D.; A hormone pulse induces transient changes in the subcellular distribution and leads to a lysosomal accumulation of the estradiol receptor alpha in target tissues. Eur. J. Cell Biol., 79, 383-93 (2000).
  35. Rayner, S. L., and Stephenson, F. A. Labelling and characterization of gamma-amminobutyric acidA receptor subunit-specific antibodies with monomaleimido-Nanogold. Biochem. Soc. Trans., 25, 546S (1997).
  36. Sawada, H., and Esaki, M.: A practical technique to postfix Nanogold-immunolabeled specimens with osmium and to embed them in Epon for electron microscopy J. Histochem. Cytochem., 48, 493-498 (2000).
  37. Sawada, H., and Esaki, H: Use of Nanogold followed by silver enhancement and gold toning for preembedding immunolocalization. J. Elect. Micro., 43, 361-66 (1994).
  38. Soussan, L.; Burakov, M.; Daniels, M. P.; Toister-Achituv, M.; Porat, A.; Yarden, Y., and Elazar, Z.: ERG30, a VAP-33-related protein, functions in protein transport mediated by COPI vesicles. J. Cell Biol., 146, 301-311 (1999).
  39. Suzuki, Y.; Itakura, M.; Kashiwagi, M.; Nakamura, N.; Matsuki, T.; Sakuta, H.; Naito, N.; Takano, K.; Fujita, T., and Hirose, S.: Identification by differential display of a hypertonicity-inducible inward rectifier potassium channel highly expressed in chloride cells. J. Biol. Chem, 274, 11376-11382 (1999).
  40. Takizawa, T.: High-resolution Immunocytochemical Labeling of Replicas with Ultrasmall Gold. J. Histochem. Cytochem., 47, 569-573 (1999).
  41. Thompson, W. F., Beven, A. F., Wells, B., and Shaw, P. J. Sites of rDNA transcription genes are widely dispersed through the nucleolus in Pisum sativum and can comprise single genes. Plant J., 12, 571-581 (1997).
  42. Tolstonog, G. V.; Sabasch, M., and Traub, P.: Cytoplasmic Intermediate Filaments Are Stably Associated with Nuclear Matrices and Potentially Modulate Their DNA-Binding Function. DNA Cell Biol., 21, 213-39 (2002).
  43. Yanase, K.; Smith, R. M.; Cizman, B.; Foster, M. H.; Peachey, L. D.; Jarrett, L., and Madaio, M. P.: A subgroup of Murine monoclonal anti-deoxyribonucleic acid antibodies traverse the cytoplasm and enter the nucleus in a time- and temperature- dependent manner; Laboratory Investigation, 71, 52-60 (1994).
  44. Weipoltshammer, K.; Schéfer, C.; Almeder, M., and Wachtler, F.: Signal enhancement at the electron microscopic level using Nanogold and gold-based autometallography. Histochem. Cell Biol., 114, 489-495 (2000).
  45. Yoshimori, T.; Yamagata, F.; Yamamoto, A.; Mizushima, N.; Kabeya, Y.; Nara, A.; Miwako, I.; Ohashi, M.; Ohsumi, M., and Ohsumi, Y.: The Mouse SKD1,a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells Mol. Biol. Cell, 11, 747-763 (2000).

 

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Gels: Staining Nanogold® Labeled Proteins on Gels

  1. Gregori, L., Hainfeld, J. F., Simon, M. N., and Goldgaber, D. Binding of amyloid beta protein to the 20S proteasome. J. Biol. Chem., 272, 58-62 (1997).
  2. Hainfeld, J. F., and Furuya, F. R.: Silver Enhancement of Nanogold and Undecagold. In Immunogold-Silver Staining: Principles, Methods and Applications," M. A. Hayat (Ed.); CRC Press, Boca Raton, FL, 1995, pp. 71-96.
  3. Rayner, S. L., and Stephenson, F. A. Labelling and characterization of gamma-amminobutyric acidA receptor subunit-specific antibodies with monomaleimido-Nanogold. Biochem. Soc. Trans., 25, 546S (1997).

 

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In Situ Hybridization and In Situ PCR

  1. Cheung, A. L.; Graf, A. H.; Hauser-Kronberger, C.; Dietze, O.; Tubbs, R. R., and Hacker, G. W.: Detection of human papillomavirus in cervical carcinoma: comparison or peroxidase, Nanogold, and catalyzed reporter deposition (CARD)-Nanogold in situ hybridization Mod. Pathol., 12, 689 (1999).
    • Gerhard W. Hacker: Medline search (results will be displayed in a new window).
  2. Graf, A. H.; Cheung, A. L.; Hauser-Kornberger, C.; Dandachi, N.; Tubbs, R. R.; Dietze, O., and Hacker, G. W.: Clinical relevance of HPV 16/18 testing methods in cervical squamous cell carcinoma. Appl. Immunohistochem. Molecul. Morphol., 8, 300-9 (2000).
  3. Hacker, G. W.; Hauser-Kronberger, C.; Zehbe, I.; Su, H., and Tubbs, R.: New advances in super-sensitive DNA-, RNA- and antigen detection: Combination of labeled tyramides with Nanogold-silver staining (NGSS). Proc. 56th Ann. Mtg., Micros. Soc. Amer.; Bailey, G. W.; Alexander, K. B.; Jerome, W. G.; Bond, M. G., and McCarthy, J. J., Eds.; Springer, New York, NY, 1998, 996-997.
  4. Hacker, G. W., Hauser-Kronberger, C., Zehbe, I., Su, H., Schiechl, A., Dietze, O., and Tubbs, R.: In Situ localization of DNA and RNA sequences: Super-sensitive In Situ hybridization using Streptavidin-Nanogold®-Silver Staining: Minireview, Protocols and Possible Applications. Cell Vision, 4, 54-65 (1997).
  5. Hacker, G. W.; Zehbe, I.; Hainfeld, J.; Sällström, J.; Hauser-Kronberger, C.; Graf, A.-H.; Su, H.; Dietze, O., and Bagasra, O; High-Performance Nanogold® In Situ Hybridization and In Situ PCR. Cell Vision, 3, 209 (1996).
  6. Hacker, G. W., Zehbe, I., Hainfeld, J., Graf, A.-H., Hauser-Kronberger, C., Schiechl, A., Su, H., and Dietze, O.: High performance Nanogold®-in situ hybridization and its use in the detection of hybridized and PCR-amplified microscopical preparations; In Proc 54th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, J. M. Corbett, R. V. W. Dimlich, J. R. Michael and N. J., Zaluzec (Eds.). San Francisco Press, San Francisco, CA, pp. 896-897 (1996).
  7. Hauser-Kronberger, C.: Highly sensitive DNA, RNA and antigen detection methods. Streptavidin-Nanogold-silver staining. Cell Vision, 5, 83 (1998).
  8. Punnonen, E.-L., Fages, C., Wartiovaara, J., and Rauvala, H.: Ultrststructural Localization of beta-Actin and Amphoterin mRNA in Cultured Cells: Application of tyramide signal amplification and comparison of detection methods; J. Histochem. Cytochem., 47, 99 (1999).
  9. Schöfer, C.; Weipoltshammer, K.; Hauser-Kronberger, C., and Wachtler, F.: High-resolution detection of nucleic acids at the electron microscope level - Review of in situ hybridization technology, the use of gold, and Catalyzed Reporter Deposition (CARD). Cell Vision, 4, 443-454 (1997).
  10. Tbakhi, A.; Totos, G.; Hauser-Kronberger, C.; Pettay, J.; Baunoch, D.; Hacker, G. W., and Tubbs, R. R.: Fixation conditions for DNA and RNA in situ hybridization: a reassessment of molecular morphology dogma. Am. J. Pathol., 152, 35-41 (1998).
  11. Totos, G.; Tbakhi, A.; Hauser-Kronberger, C., and Tubbs, R. R.: Catalyzed Reporter Deposition: A new era in molecular and immunomorphology - Nanogold-silver staining and colorimetric detection and protocols. Cell Vision, 4, 433-442 (1997).
  12. Tubbs, R.; Pettay, J.; Skacel, M.; Powell, R.; Stoler, M.; Roche, P., and Hainfeld, J.: Gold-Facilitated in Situ Hybridization: A Bright-Field Autometallographic Alternative to Fluorescence in Situ Hybridization for Detection of HER-2/neu Gene Amplification. Am. J. Pathol., 160, 1589-1595 (2002).
  13. Weipoltshammer, K.; Schéfer, C.; Almeder, M., and Wachtler, F.: Signal enhancement at the electron microscopic level using Nanogold and gold-based autometallography. Histochem. Cell Biol., 114, 489-495 (2000).
  14. Zehbe, I., Hacker, G.W., Su, H., Hauser-Kronberger, C., Hainfeld, J.F., and Tubbs, R.: Sensitive in situ hybridization with catalyzed reporter deposition, streptavidin-Nanogold, and silver acetate autometallography. Detection of single-copy human papillomavirus. Am. J. Pathol. 150, 1553-1561 (1997).

 

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Light Microscopy and Confocal Microscopy

  1. Griffing, L. R., Villanueva, M. A., Taylor, J., and Moon, S.: Confocal Epipolorization Microscopy of Gold Probes in Plant Cells and Protoplasts; Methods in Cell Biology, 49, 109-121 (1995).
  2. Kohler, A.; Lauritzen, B., and Van Noorden, J. F.: Signal amplification in immunohistochemistry at the light microscopic level using biotinylated tyramide and Nanogold-silver staining J. Histochem. Cytochem., 48, 933-941 (2000).
  3. Lackie, P. M.: Immunogold silver staining for light microscopy; Histochem. Cell. Biol., 106, 9-17 (1996).
  4. Ribrioux, S., Kleymann, G., Haase, W., Heitmann, K., Ostermeier, C., and Michel, H. Use of Nanogold- and Fluorescent-labeled Antibody Fv Fragments in Immunocytochemistry. J. Histochem. Cytochem., 44, 207-213 (1996).
  5. Segond von Banchet, G., and Heppelman, B.: Non-radioactive localization of substance P binding sites in rat brain and spinal cord using peptides labeled with 1.4 nm gold particles; J. Histochem. Cytochem., 43, 821 (1995).
  6. Sun, X.J., Tolbert, L.P. and Hildebrand, J.G. Using laser scanning confocal microscopy as a guide for electron microscopic study: a simple method for correlation of light and electron microscopy. J. Histochem. Cytochem., 43, 329-35 (1995).

 

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Molecular Transmission Electron Microscopy

Cryo-electron Microscopy

  1. Aoki, T.; Hagiwara, H., and Fujimoto, T.: Peculiar Distribution of Fodrin in Fat-Storing Cells; Exp. Cell. Res., 234, 313-320 (1997).
  2. Boisset, N., Penczek, P., Pochon, F., Frank, J., and Lamy, J. Three-dimensional reconstruction of human alpha 2-macroglbulin and refinement of the localization of thiol ester bonds with monomaleimido Nanogold;. Ann. NY Acad. Sci., 737, 229-44 (1994).
  3. Boisset, N., Grassucci, R., Penczek, P., Delain, E., Pochon, F., Frank, J., and Lamy, J.N. Three-dimensional reconstruction of a complex of human alpha-2-macroglobulin with monomaleimido Nanogold; (Au1.4nm) embedded in ice. J. Struct. Biol., 109;39-45 (1992).
  4. Jeon, H., and Shipley, G. G. Localization of the N-Terminal Domain of the Low Density Lipoprotein Receptor. J. Biol. Chem.,, 275, 30465-30470 (2000).
  5. Jeon, H., and Shipley, G. G. Vesicle-Reconstituted Low Density Lipoprotein Receptor: Visualization by Cryoelectron Microscopy. J. Biol. Chem., 275, 30458-30464 (2000).
  6. Montesano-Roditis, L.; Glitz, D. G.; Traut, R. R., and Stewart, P. L.: Cryo-electron microscopic localization of protein L7/L12 within the Escherichia coli 70S ribosome by difference mapping and Nanogold labeling. J. Biol. Chem., e-publication ahead of print.
  7. Wagenknecht, T,; Berkowitz, J.; Grassucci, R.; Timerman, A. P., and Fleischer, S.: Localization of calmodulin binding sites on the ryanodine receptor from skeletal muscle by electron microscopy. Biophys. J., 67, 2286-2295 (1994).
  8. Wilkens, S. and Capaldi, R.A. Monomaleimidogold Labeling of the g subunit of the E. coli F1 ATPase examined by cryoelectron Microscopy. Arch Biochem. Biophys., 229, 105-109 (1992).
  9. Woldin, C. N.; Hing, F. S.; Lee, J.; Pilch, P. F., and Shipley, G. G.: Structural studies of the detergent-solubilized and vesicle-reconstituted insulin receptor J. Biol. Chem, 274, 34981-34992 (1999).

 

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Molecular TEM: Staining

  1. Scheibel, T.; Kowal, A. S.; Bloom, J. D., and Lindquist, S.L.: Bidirectional amyloid fiber growth for a yeast prion determinant. Current Biology, 11, 366-369 (2001).
  2. Wenzel, T., and Baumeister, W.: Conformational restraints in protein degradation by the 20S proteasome. Nature Struct. Biol., 2, 199-204 (1995).

 

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Peptide Labeling

  1. Gregori, L., Hainfeld, J. F., Simon, M. N., and Goldgaber, D. Binding of amyloid beta protein to the 20S proteasome. J. Biol. Chem., 272, 58-62 (1997).
  2. Larrson, L.-I.: Immunogold labeling of neuroendocrine peptides with special reference to antibody specificity and multiple staining techniques [review]; Histochem. Cell. Biol., 106, 93-103 (1996).
  3. Malecki, M.; Hsu, A.; Truong, L., and Sanchez, S: Molecular immunolabeling with recombinant single-chain variable fragment (scFv) antibodies designed with metal-binding domains; Proc. Natl. Acad. Sci. USA, 99, 213-218 (2002).
  4. Ribrioux, S., Kleymann, G., Haase, W., Heitmann, K., Ostermeier, C., and Michel, H. Use of Nanogold- and Fluorescent-labeled Antibody Fv Fragments in Immunocytochemistry. J. Histochem. Cytochem., 44, 207-213 (1996).
  5. Segond von Banchet, G., Schindler, M., Hervieu, G. J.; Beckmann, B., Emson, P. C., and Heppelmann, B.: Distribution of somatostain receptor subtypes in rat lumbar spinal cord examined with gold-labelled somatostatin and anti-receptor antibodies; Brain Res., 816, 254 (1999).
  6. Segond von Banchet, G., and Heppelman, B.: Non-radioactive localization of substance P binding sites in rat brain and spinal cord using peptides labeled with 1.4 nm gold particles; J. Histochem. Cytochem., 43, 821 (1995).
  7. Wenzel, T., and Baumeister, W.: Conformatinal restraints in protein degradation by the 20S proteasome. Nature Struct. Biol., 2, 199-204 (1995).
  8. Wagenknecht, T.; Berkowitz, J.; Grassucci, R.; Timerman, A. P., and Fleischer, S.; Localization of Calmodulin Binding Sites on the Ryanodine Receptor from Skeletal Muscle by Electron Microscopy. Biophys. J., 67, 2286-2295 (1994).

 

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Replicas

  1. Kloboucek, A.; Behrisch, A.; Faix, J., and Sackmann, E.: Adhesion-induced receptro segregation and adhesion plaque formation: a model membrane study. Biophys. J., 77, 2311-2328 (1999).
  2. Takizawa, T.: High-resolution Immunocytochemical Labeling of Replicas with Ultrasmall Gold. J. Histochem. Cytochem., 47, 569-573 (1999).

 

Nanogold® Labeling Reagents: Catalog Information | Technical Help | Product Instructions

 

 

SEM - Scanning Electron Microscopy

  1. Hermann, R., Walther, P., and Muller, M.: Immunogold labeling in scanning electron microscopy; Histochem. Cell Biol., 106, 31-39 (1996).

 

Nanogold® Labeling Reagents: Catalog Information | Technical Help | Product Instructions

 

Sizing

  1. Schwartz, M. P., and Matouschek, A.: The dimensions of the protein import channels in the outer and inner mitochondrial membranes. Proc. Natl. Acad. Sci. USA, 96, 13086-13090 (1999).

 

Nanogold® Labeling Reagents: Catalog Information | Technical Help | Product Instructions

 

STEM: Scanning Transmission Electron Microscopy

  1. Braig, K., Simon, M., Furuya, F., Hainfeld, J.F., and Horwich, A.L. A polypeptide bound by the chaperonin groEL is localized within a central cavity. Proc. Natl. Acad.Sci., 90, 3978-3982 (1993).
  2. Traxler, K. W.; Norcum, M. T.; Hainfeld, J. F., and Carlson, G. M.: Direct Visualization of the Calmodulin Subunit of Phosphorylase Kinase via Electron Microscopy Following Subunit Exchange. J. Struct. Biol., 135, 231-8 (2001).
  3. Ottensmeyer, F. P.; Luo, R. Z.-T.; Fernandes, A. B.; Beniac, D., and Yip, C. C.: Insulin receptor: 3D reconstruction from darkfield STEM images, structural interpretation and functional model; Proc. 57th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 408-409.
  4. Wall, J. S.: Visualizing "Greengold" clusters in the STEM. J. Struct. Biol., 127, 161-168 (1999).
  5. Yang, Y. S.; Datta, A.; Hainfeld, J. F.; Furuya, F. R.; Wall, J. S., and Frey, P. A.: Mapping the lipoyl groups of the pyruvate dehydrogenase complex by use of gold cluster labels and scanning transmission electron microscopy. Biochemistry, 16;33(32), 9428-9437 (1994).

 

Nanogold® Labeling Reagents: Catalog Information | Technical Help | Product Instructions

 

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