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References: 1.4 nm Nanogold® Conjugates

 

 

 

Nanogold® Antibody Conjugates

  1. Jose J. Ferrero, Ana M. Alvarez, Jorge Ramírez-Franco, María C. Godino, David Bartolomé-Martín, Carolina Aguado, Magdalena Torres, Rafael Luján, Francisco Ciruela and José Sánchez-Prieto. β-Adrenergic Receptors Activate Exchange Protein Directly Activated by cAMP (Epac), Translocate Munc13-1, and Enhance the Rab3A-RIM1α Interaction to Potentiate Glutamate Release at Cerebrocortical Nerve Terminals. J. Biol. Chem. 2013 288: 31370-31385.
  2. Jennifer L Ziskin, Akiko Nishiyama, Maria Rubio,2 Masahiro Fukaya, and Dwight E Bergles. Vesicular release of glutamate from unmyelinated axons in white matter. Nat Neurosci. 2007 March; 10(3): 321–330.
  3. Tolstonog, G. V.; Sabasch, M., and Traub, P.: Cytoplasmic Intermediate Filaments Are Stably Associated with Nuclear Matrices and Potentially Modulate Their DNA-Binding Function. DNA Cell Biol., 21, 213-39 (2002).
  4. Bendayan, M.: Worth its weight in gold. Science, 291, 1363-5 (2001).
  5. Bergles, D. E.; Roberts, J. D. B.; Somogyi, P., and Jahr, C. E.: Glutamatergic synapses on oligodendrocyte precursor cells in the hippocampus. Nature, 405, 187-190 (2000).
  6. D'Este, L.; Kulaksiz, H.; Rausch, U.; Vaccaro, R.; Wenger, T.; Tokunaga, Y.; Renda, T. G.; Cetin, Y.: Expression of guanylin in "pars tuberalis-specific cells" and gonadotrophs of rat adenohypophysis Proc. Natl. Acad. Sci. USA, 97, 1131-1136 (2000).
  7. Feng, D.; Nagy, J. A.; Brekken, R. A.; Pettersson, A.; Manseau, E. J.; Pyne, L.; Mulligan, R.; Thorpe, P. E.; Dvorak, H. F., and Dvorak, A. M.: Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF-expressing tumors and adenoviral vectors J. Histochem. Cytochem., 48, 545-555 (2000).
  8. Grondin, G., and Beaudoin, A. R.: A New Pre-Embedding Immunogold Method that Permits to Obtain a Very High Signal with a Very Good Ultrastructure. Microsc. Microanal., 7, (Suppl. 2: Proceedings) (Proceedings of the Fifty-Ninth Annual Meeting, Microscopy Society of America); Bailey, G. W.; Price, R. L.; Voelkl, E., and Musselman, I. H., Eds.; Springer-Verlag, New York, NY, 2001, pp. 1044-1045.
  9. Hainfeld, J. F.: Labeling with Nanogold and undecagold: techniques and results. Scanning Microsc. Suppl. (Proc. 14th Pfefferkorn Conf.); Malecki, M., and Roomans, G. M. (Eds.). Scanning Microscopy International, Chicago, IL, 10, 309-322 (1996).
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  11. Kohler, A.; Lauritzen, B., and Van Noorden, J. F.: Signal amplification in immunohistochemistry at the light microscopic level using biotinylated tyramide and Nanogold-silver staining J. Histochem. Cytochem., 48, 933-941 (2000).
  12. Malecki, M.: Preparation of plasmid DNA in transfection complexes for fluorescence and spectroscopic imaging. Scanning Microsc. Suppl. (Proc. 14th Pfefferkorn Conf.); Malecki, M., and Roomans, G. M. (Eds.). Scanning Microscopy International, Chicago, IL, 10, 1-16 (1996).
  13. Robinson, J. M.; Takizawa, T., and Vandré, D. D.: Applications of gold cluster compounds in immunocytochemistry and correlative microscopy: comparison with colloidal gold. J. Microsc., 199, 163-79 (2000).
  14. Robinson, J. M.; Takizawa, T., and Vandré: Enhanced immunolabeling efficiency using ultrasmall immunogold probes: Immunocytochemistry J. Histochem. Cytochem., 48, 487-492 (2000).
  15. Sawada, H., and Esaki, M.: A practical technique to postfix Nanogold-immunolabeled specimens with osmium and to embed them in Epon for electron microscopy J. Histochem. Cytochem., 48, 493-498 (2000).
  16. Yang, R.; Tabata, S.; Crowley, H. H.; Margolskee, R. F., and Kinnamon, J. C.: Ultrastructural localization of gustducin immunoreactivity in microvilli of type II taste cells in the rat. J. Comp. Neurol., 11, 139-151 (2000).
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  19. Ackerly, C. A., Becker, L. E., Tilups, A., Rutlka, J. T., and Mancuso, J. F.: CCD Cameras facilitate the imaging of small gold particles in immunogold-labelled ultrathin cryosections. In Proc 54th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, J. M. Corbett, R. V. W. Dimlich, J. R. Michael and N. J., Zaluzec (Eds.). San Francisco Press, San Francisco, CA, pp. 904-905 (1996).
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  24. Bernard, V.; Levey, A. I., and Bloch, B.: Regulation of the subcellular distribution of m4 muscarinic acetylcholine receptors in striatal neurons in vivo by the cholinergic environment: evidence for regulation of cell surface receptors by endogenous and exogenous stimulation J. Neurosci., 19, 10237-10249 (1999).
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  28. Burry, R.W., Vandrée, D. D., and Hayes, D. M.: Silver enhancement of gold antibody probes in pre-embedding electron microscopic immunocytochemistry. J. Histochem. Cytochem., 40, 1849-1856 (1992).
  29. Du, J.; Tao-Cheng, J.-H.; Zerfas, P., and McBain, C. J. The K+ channel, Kv2.1, is apposed to astrocytic processes and is associated with inhibitory postsynaptic membranes in hippocampal and cortical principal neurons and inhibitory interneurons. Neuroscience, 84, 37-48 (1998).
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  34. Gilerovitch, H.G., Bishop, G.A., King, J.S., and Burry, R.W. The use of electron microscopic immunocytochemistry with silver-enhanced 1.4nm gold particles to localize GAD in the cerebellar nuclei. J. Histochem. Cytochem., 43, 337-43 (1995).
  35. Griffing, L. R., Villanueva, M. A., Taylor, J., and Moon, S.: Confocal Epipolorization Microscopy of Gold Probes in Plant Cells and Protoplasts; Methods in Cell Biology, 49, 109-121 (1995).
  36. Gutekunst, C.-A.; Li, S.-H.; Yi, H.;Mulroy, J. S.; Kuemmerle, S.; Jones, R.; Rye, D.; Ferrante, R. J.; Hersch, S. M., and Li, X.-J.: Nuclear and Neuropil Aggregates in Huntington's Disease: Relationship to Neuropathology. J. Neuroscience, 19, 2522-2534 (1999).
  37. Hainfeld, J.F. Site specific cluster labels. Ultramicroscopy, 46, 135-144 (1992).
  38. Hainfeld, J. F., and Furuya, F. R.: Silver-enhancement of Nanogold and undecagold: in Immunogold-Silver Staining: Principles, Methods and Applications," M. A. Hayat (Ed.); CRC Press, Boca Raton, FL, 1995, pp. 71-96.
  39. Hainfeld, J.F. and Furuya, F.R. A 1.4nm Gold cluster covalently attached to antibodies improves immunolabeling, J. Histochem. Cytochem., 40, 177-184 (1992).
  40. Hainfeld, J. F., and Powell, R. D.: Nanogold Technology: New Frontiers in Gold Labeling. Cell Vision, 4, 408-432 (1997).
  41. Halasy, K.; Buhl, E. H.; Lörinczi, Z.; Tamás, G., and Somogyi, P: Synaptic target selectivity and input of GABAergic basket and bistratified interneurons in the CA1 area of the rat hippocampus. Hippocampus, 6, 306-329 (1996).
  42. Hanson, J. E., and Smith, Y.: Group I metabotropic glutamate receptors at GABAergic synapses in monkeys; J. Neurosci., 19, 6488-6496 (1999).
  43. Hearne, C. E.; Johnson, D. L., and Van Campen, H.: Immunogold-Silver Staining (IGSS) of (NCP and CP) BVDV-infected subcellular fraction bands; In Proc 55th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, R. V. W. Dimlich, K. B. Alexander, J. J. McCarthy and T. P. Pretlow (Eds.). Springer-Verlag, New York, NY, pp. 155-156 (1997).
  44. Hearne, C. E., and Van Campen, H.: Immunogold-Silver Staining (IGSS) of agarose embedded (NCP) BVDV-infected cell suspensions; In Proc 54th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, J. M. Corbett, R. V. W. Dimlich, J. R. Michael and N. J., Zaluzec (Eds.). San Francisco Press, San Francisco, CA, pp. 902-903 (1996).
  45. Hermann, R., Walther, P., and Muller, M.: Immunogold labeling in scanning electron microscopy; Histochem. Cell Biol., 106, 31-39 (1996).
  46. Humbel, B. M.; De Jong, M. D. M.; Müller, W. H., and Verkleij, A. J.: Pre-embedding immunolabeling for electron microscopy: An evaluation of permeabilization methods and markers. Microsc. Res. Tech., 42, 43-58 (1998).
  47. Humbel, B. M.; Sibon, O. C. M.; Stierhof, Y.-D., and Schwarz, H.: Ultra-small gold particles and silver enhancement as a detection system in immunolabeling and In Situ hybridization experiments; J. Histochem. Cytochem., 43, 735-737 (1995).
  48. Kloboucek, A.; Behrisch, A.; Faix, J., and Sackmann, E.: Adhesion-induced receptor segregation and adhesion plaque formation: a model membrane study. Biophys. J., 77, 2311-2328 (1999).
  49. Krenács, T., and Krenács, L.: Immunogold-silver staining (IGSS) for single and multiple antigen detection in archived tissues following antigen retrieval. Cell Vision, 4, 387-393 (1997).
  50. Krenács, T. and Dux, L. Silver-enhanced immunogold labeling of calcium-ATPase in sarcoplasmic reticulum of skeletal muscle. J. Histochem. Cytochem., 42, 967-968 (1994).
  51. Lackie, P. M.: Immunogold silver staining for light microscopy; Histochem. Cell. Biol., 106, 9-17 (1996).
  52. Larrson, L.-I.: Immunogold labeling of neuroendocrine peptides with special reference to antibody specificity and multiple staining techniques [review]; Histochem. Cell. Biol., 106, 93-103 (1996).
  53. Li, H.; Ohishi, H.; Kinoshita, A.; Shigemoto, R.; Nomura, S., and Mizuno, N.: Localization of a metabotropic glutamate receptor, mGluR7, in axon terminals of presumed nociceptive, primary afferent fibers in the superficial layers of the spinal dorsal horn: an electron microscope study in the rat. Neuroscience lett., 223, 153-156 (1997).
  54. Li, S.-H.; Cheng, A. L.; Li, H., and Li, X.-J.: Cellular effects and altered gene expression in PC12 cells stably expressing mutant huntingtin. J. Neuroscience, 19, 5159-5172 (1999).
  55. Lipschitz, D. L., and Michel, W. C.: Physiological Evidence for the discrimination of L-arginine from structural analogues by the zebrafish olfactory system J. Neurophysiol., 82, 3160-3167 (1999).
  56. Lujan, R.; Nusser, Z.; Roberts, J. D. B.; Shigemoto R.; Ohishi, H., and Somogyi, P.: Differential plasma membrane distribution of metabotropic glutamate receptors mGluR1-alpha, mGluR1 and mGluR5, relative to neurotransmitter release sites. J. Chem. Neuroanat., 13, 219-241 (1997).
  57. Lujan, R.; Nusser, Z.; Roberts, J. D. B.; Shigemoto R., and Somogyi, P.: Perisynaptic location of metabotropic glutamate receptors mGluR1 and mGluR5 on dendrites and dendritic spines in the rat hippocampus. Eur. J. Neuroscience, 8, 1488-1500 (1996).
  58. Matsubara, A., Laake, J. H., Davanger, S., Usami, S.-I., and Otterson, O. P.; Organization of AMPA receptor subunits at a glustamate synapse: A quantitative immunogold analysis of hair cell synapses in the rat organ of Corti. J. Neuroscience, 16, 4457-4467 (1996).
  59. Matsuzaki, T.; Suzuki, T.; Koyama, H.; Tanaka, S., and Takata, K.: Water channel protein AQP3 is present in epithelia exposed to the environment of possible water loss. J. Histochem. Cytochem., 47, 1275-1286 (1999).
  60. Mizoguchi, A.; Yano, Y.; Hamaguchi, H.; Yanagida, H.; Ide, C.; Zahraoui, A.; Shirataki, H.; Sasaki, T., and Takai, Y.: Localization of Rabphilin-3A on the synaptic vesicle. Biochem. Biophys. Res. Commun, 202, 1235-1243 (1999).
  61. Nixon, G. F., Mignery, G. A., and Somlyo, A. V.: Immunogold localization of inositol 1,4,5-trisphosphate receptors and characterization of ultrastructural features of the sarcoplasmic reticulum in phasic and tonic smooth muscle; J. Muscle Res. Cell Mot., 15, 682-700 (1994).
  62. Nusser, Z.; Cull-Candy, S., and Farrant, M.; Differences in synaptic GABAA receptor number underlie variation in GABA mini amplitude; Neuron, 19, 697-709 (1997).
  63. Nusser, Z.; Hajos, N.; Somogyi, P., and Mody, I.: Increased number of synaptic GABAA receptors underlies potentiation at hippocampal inhibitory synapses; Nature, 395, 172-177 (1998).
  64. Nusser, Z.; Mulvihill, E.; Streit, P., and Somogyi, P.: Subsynaptic segregation of metabotropic and ionotropic glutamate receptors as revealed by immunogold localization. Neuroscience, 61, 421-427 (1994).
  65. Nusser, Z.; Roberts, J. D. B.; Baude, A.; Richards, J. G., and Somogyi, P. Relative densities of synaptic and extrasynaptic GABA-A receptors on cerebellar granule cells as determined by a quantitative immunogold method. J. Neuroscience; 15, 2948-2960 (1995).
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  67. Nusser, Z.; Sieghart, W.; Benke, D.; Fritschy, J.-M., and Somogyi, P.: Differential synaptic localization of two major gamma-aminobutyric acid type A receptor alpha subunits on hippocampal pyramidal cells. Proc. Natl. Acad. Sci. USA, 93, 11939-11944 (1996).
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  69. Nusser, Z.; Sieghart, W.; Stephenson, F. A., and Somogyi, P.: The alpha-6 subunit of the GABAA receptor is concentrated in both inhibitory and excitatory synapses on cerebellar granule cells. J. Neuroscience, 16, 103-114 (1996).
  70. Nusser, Z., and Somogyi, P.: Compartmentalised distribution of GABAA and glutamate receptors in relation to transmitter release sites on the surface of cerebellar neurones. Prog. Brain Research, (C. I. De Zeeuw, P. Strata and J. Voogd, Eds.), Elsevier, Oxford, UK.; 114, 1488-1500 (1997).
  71. Pohl, K., and Stierhof, Y.-D.: Action of gold chloride ("Gold toning") on silver-enhanced 1 nm gold markers. Microsc. Res. Tech., 42, 66-79 (1998).
  72. Punnonen, E.-L., Fages, C., Wartiovaara, J., and Rauvala, H.: Ultrststructural Localization of beta-Actin and Amphoterin mRNA in Cultured Cells: Application of tyramide signal amplification and comparison of detection methods; J. Histochem. Cytochem., 47, 99 (1999).
  73. Robinett, C. C., Straight, A., Li, G., Willhelm, C., Sudlow, G., Murray, A., and Belmont, A. S.: In vivo localization of DNA sequences and visualization of large-scale chromatin organization using lac operator/repressor recognition. J. Cell Biol., 135, 1685-1700 (1996).
  74. Robinson, J. M., and Takizawa, T.: Novel Labeling methods for EM analysis of ultrathin cryosections. In Proc 54th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, J. M. Corbett, R. V. W. Dimlich, J. R. Michael and N. J., Zaluzec (Eds.). San Francisco Press, San Francisco, CA, pp. 894-895 (1996).
  75. Salas, P. J. I.: Insoluble gamma-tubulin-containing structures are anchored to the apical network of intermediate filaments in polarized CACO-2 epithelial cells. J. Cell Biol., 146, 645-657 (1999).
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Nanogold® Conjugates: catalog information | Tech Help | Product Instructions

 

 

Nanogold® Streptavidin

  1. Bendayan, M.: Worth its weight in gold. Science, 291, 1363-5 (2001).
  2. Graf, A. H.; Cheung, A. L.; Hauser-Kornberger, C.; Dandachi, N.; Tubbs, R. R.; Dietze, O., and Hacker, G. W.: Clinical relevance of HPV 16/18 testing methods in cervical squamous cell carcinoma. Appl. Immunohistochem. Molecul. Morphol., 8, 300-9 (2000).
    • Gerhard Hacker: Medline search (results will be displayed in a new window).
  3. Tubbs, R.; Pettay, J.; Skacel, M.; Powell, R.; Stoler, M.; Roche, P., and Hainfeld, J.: Gold-Facilitated in Situ Hybridization: A Bright-Field Autometallographic Alternative to Fluorescence in Situ Hybridization for Detection of HER-2/neu Gene Amplification. Am. J. Pathol., 160, 1589-1595 (2002).
  4. Weipoltshammer, K.; Schéfer, C.; Almeder, M., and Wachtler, F.: Signal enhancement at the electron microscopic level using Nanogold and gold-based autometallography. Histochem. Cell Biol., 114, 489-495 (2000).
  5. Cheung, A. L.; Graf, A. H.; Hauser-Kronberger, C.; Dietze, O.; Tubbs, R. R., and Hacker, G. W.: Detection of human papillomavirus in cervical carcinoma: comparison or peroxidase, Nanogold, and catalyzed reporter deposition (CARD)-Nanogold in situ hybridization Mod. Pathol., 12, 689 (1999).
    • Gerhard Hacker: Medline search (results will be displayed in a new window).
  6. Hacker, G. W., and Danscher, G.: Recent Advances in Immunogold-Silver Staining-Autometallography; Cell Vision 1, 102 (1994).
  7. Hacker, G. W., Hauser-Kronberger, C., Zehbe, I., Su, H., Schiechl, A., Dietze, O., and Tubbs, R.: In Situ localization of DNA and RNA sequences: Super-sensitive In Situ hybridization using Streptavidin-Nanogold-Silver Staining: Minireview, Protocols and Possible Applications. Cell Vision, 4, 54-65 (1997).
  8. Hacker, G. W.; Hauser-Kronberger, C.; Zehbe, I.; Su, H., and Tubbs, R.: New advances in super-sensitive DNA-, RNA- and antigen detection: Combination of labeled tyramides with Nanogold-silver staining (NGSS). Proc. 56th Ann. Mtg., Micros. Soc. Amer.; Bailey, G. W.; Alexander, K. B.; Jerome, W. G.; Bond, M. G., and McCarthy, J. J., Eds.; Springer, New York, NY, 1998, 996-997.
  9. Hacker, G. W.; Zehbe, I.; Hainfeld, J.; Sällström, J.; Hauser-Kronberger, C.; Graf, A.-H.; Su, H.; Dietze, O., and Bagasra, O; High-Performance Nanogold® In Situ Hybridization and In Situ PCR. Cell Vision, 3, 209 (1996).
  10. Hacker, G. W., Zehbe, I., Hainfeld, J., Graf, A.-H., Hauser-Kronberger, C., Schiechl, A., Su, H., and Dietze, O.: High performance Nanogold®-in situ hybridization and its use in the detection of hybridized and PCR-amplified microscopical preparations; In Proc 54th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, J. M. Corbett, R. V. W. Dimlich, J. R. Michael and N. J., Zaluzec (Eds.). San Francisco Press, San Francisco, CA, pp. 896-897 (1996).
  11. Hauser-Kronberger, C.: Highly sensitive DNA, RNA and antigen detection methods. Streptavidin-Nanogold-silver staining. Cell Vision, 5, 83 (1998).
  12. Schöfer, C.; Weipoltshammer, K.; Hauser-Kronberger, C., and Wachtler, F.: High-resolution detection of nucleic acids at the electron microscope level - Review of in situ hybridization technology, the use of gold, and Catalyzed Reporter Deposition (CARD). Cell Vision, 4, 443-454 (1997).
  13. Tbakhi, A.; Totos, G.; Hauser-Kronberger, C.; Pettay, J.; Baunoch, D.; Hacker, G. W., and Tubbs, R. R.: Fixation conditions for DNA and RNA in situ hybridization: a reassessment of molecular morphology dogma. Am. J. Pathol., 152, 35-41 (1998).
  14. Totos, G.; Tbakhi, A.; Hauser-Kronberger, C., and Tubbs, R. R.: Catalyzed Reporter Deposition: A new era in molecular and immunomorphology - Nanogold-silver staining and colorimetric detection and protocols. Cell Vision, 4, 433-442 (1997).
  15. Woldin, C. N.; Hing, F. S.; Lee, J.; Pilch, P. F., and Shipley, G. G.: Structural studies of the detergent-solubilized and vesicle-reconstituted insulin receptor J. Biol. Chem, 274, 34981-34992 (1999).
  16. Zehbe, I., Hacker, G.W., Su, H., Hauser-Kronberger, C., Hainfeld, J.F., and Tubbs, R.: Sensitive in situ hybridization with catalyzed reporter deposition, streptavidin-Nanogold, and silver acetate autometallography. Detection of single-copy human papillomavirus. Am. J. Pathol. 150, 1553-1561 (1997).

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