Nanogold-Peptides

Peptides Linked to Nanogold® Maintain Biologic Activity

We've got Nanogold Reagents for labeling peptides-- order online! Choose from common preparations, or design your own.

30 micrometer cryostat section of rat spinal cord labeled with Nanogold- Substance P (SP is an 11 amino acid peptide) showing binding sites of this bioactive peptide. m=motoneurons, x=lamina, I+II=lamina I and II. Silver enhanced; full image width 5 microns.

Courtesey of G. Segond von Banchet and B. Heppelmann, Physiologisches Institut der Universität Würzburg, Würzburg, Germany.

Nanogold® is a small (1.4 nm) gold cluster with an organic shell and a monofunctonal reactive arm, either a maleimide (which reacts with thiols) or a sulfo-N- hydroxysuccinimide ester (which reacts with amines).

Covalent linking of the Nanogold to peptides may be achieved by reacting with the alpha-NH2 group, a lysine, a terminal cysteine that is added, or an existing cysteine.

Other Applications

• Product Applications
• Research Applications

Product Applications

• Large Covalently Linked Fluorescent and Gold Nanoparticle Immunoprobes
• Gold Labeling of Protein Fusion Tags for EM
• Special report: Very reliable pre-embedding labeling with Nanogold-Fab' and HQ Silver.
• Our 2002 paper: Nickel-NTA-Nanogold Binds his-Tagged Proteins.
• Our 2001 paper on DNA Nanowires.
• Our 1999 paper on Gold-Based Autometallography.
• Undecagold Labeling Identifies Fibrinogen Cross-Linking Site
• Special report: In Situ Hybridization with Nanogold®-Streptavidin.
• Combined Cy3 / Nanogold® Probes for Immunocytochemistry and In Situ Hybridization
• Technical note: Nanogold® Staining on Gels.
• Our 1996 paper on Preparing Gold-Labeled Liposomes.
• Special report: Nanogold® Labeling of Peptides.
• Technical note: RNA Labeling with Nanogold®.
• Our 1991 paper on A New 1.4 nm Gold-Fab' Probe.
• Our 1994 paper on Methylamine Vanadate (NanoVan) Negative Stain.
• Our 1990 paper on Conducting Polymer Films as EM Substrates.

Research Applications

• Enzymatic Metallography: A Simple New Staining Method
• Our 2007 paper on Correlative Enzymatic and Gold Probes for Light and Electron Microscopy.
• Our 2006 paper on Light and Electron Microscopy of Microsporida using Enzyme Metallography.
• Our 2004 paper on Enzymatic Metallography as a Correlative Light and Electron Microscopic Method.
• Our 2002 paper on Enzymatic Metallography: A Simple New Staining Method.
• Our 2005 paper: 5 nm Gold-Ni-NTA binds His Tags.
• Our 1995 paper on Aldehyde Gold Clusters for Molecular Labeling.
• Our 2000 paper on Gold Cluster Crystals.
• Larger covalent gold probes:
• Our 2005 paper on Preparation of Covalent 3nm Gold Fab Probes.
• Our 1999 paper on A Covalently Linked 10 nm Gold Immunoprobe.
• Combined fluorescent and gold probes:
• Our 2003 paper featuring Dextran-Texas Red®-Nanogold® Fluorescent Dyes for Use in Correlative Microscopy and Intravital Imaging.
• Our 2002 paper on Combined ALEXA-488 and Nanogold Antibody Probes.
• Our 1999 paper on Combined Cy3 / Nanogold Probes for Immunocytochemistry and In Situ Hybridization.
• Our 1998 paper on Dual-Labeled Probes for Fluorescence and Electron Microscopy.
• News: our original 1997 paper on Combined Fluorescent and Gold Immunoprobes.
• Our 1996 paper on Large Cluster and Combined Fluorescent and Gold Immunoprobes.
• Our 1994 paper on Combined Fluorescent and Gold Nucleic Acid Probes.
• Our 2005 paper on High Z Metal Carbonyls for Imaging and Microspectroscopy.
• Our 2005 paper on In Vivo Vascular Casting.