Nanogold® + Halo tags now available!
The ultimate tool for labeling proteins in live cells, now available from Nanoprobes!
Custom configuration – Your choice!
We start with a Halo tag…
…and customize to your needs
2-in-1 probe:
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Halo tag
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PLUS a Nanogold® label
Choose 1.4 nm, 5 nm or 12 nm
Precision labeling for EM!
2-in-1 Halo tag-Nanogold® custom conjugates! Available in 1.4, 5 and 12 nm – the missing link for Cryo-EM!
3-in-1 probe:
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Halo tag
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PLUS a Nanogold® label
Choose 1.4 nm, 5 nm or 12 nm
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PLUS an Alexa® fluorescent label
In the color of your choice!
3-in-1 Triple Gold Halo tags from Nanoprobes: 3-in-1 labels with 1.4 nm Nanogold®, fluorescent Alexa® 594, and a HaloTag. Penetrate the nucleus and find your target!
In the Literature
A Breathrough in Cryo-EM and Cryo-Tomography:
Identification of nuclear proteins and their structure in live cells
Recently it was reported that our Triple Gold Halo (3-in-1 Halo-Alexa®-Nanogold®) penetrated live cells, entered the nucleus, and specifically bound Halo-tagged histone variant H2AFY.
Live cells were quick frozen and the histone in situ structure elucidated by cryo-tomography.
The small 1.4 nm Nanogold® probe gets into the nucleus, and was used to identify histones and their structures in “live” cells.
Triple Nanogold® HaloTags carry a tiny, 1.4 nm gold probe, used to penetrate the nucleus and identify histones and their structure in live cells!
“…No [other] exogenous, e.g., antibodies or endogenous, e.g., small genetically encoded proteins visible in cryo-EM, have been able to specifically target and label histone-specific nucleosomes with minimal disruptions to the chromatin landscape.”
“1.4-nm Halo-Alexa-Nanogold-594, 1.4-nm Halo-Alexa-Nanogold-488, and 5-nm Halo-Nanogold conjugates were custom ordered from Nanoprobes.”
Read the full article:
Young, Lindsey N., et al. “ExoSloNano: Multi-Modal Nanogold Tags for identification of Macromolecules in Live Cells & Cryo-Electron Tomograms.” bioRxiv (2024).
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Used in this study:
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1.4-nm Halo-Alexa®-Nanogold®-594
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1.4-nm Halo-Alexa®-Nanogold®-488
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5-nm Halo-Nanogold®