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References

Explore how technology from Nanoprobes, Inc. has been used in the literature, in labs around the world.

Adler-Moore, J.: AmBisome targeting to fungal infections. Bone Marrow Transplantation, 14, S3-S7 (1994).

http://www.ncbi.nlm.nih.gov/pubmed/7703928
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Fritzsche, Florian Rudolf MD; Bode, Peter K. MD; Moch, Holger MD; Kristiansen, Glen MD; Varga, Zsuzsanna MD; Bode, Beata MD. Determination of the Her-2/neu Gene Amplification Status in Cytologic Breast Cancer Specimens Using Automated Silver-enhanced In-situ Hybridization (SISH). American Journal of Surgical Pathology: August 2010 – Volume 34 – Issue 8 – pp 1180-1185.

http://journals.lww.com/ajsp/Abstract/2010/08000/Determination_of_the_Her_2_neu_Gene_Amplification.13.aspx
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Hainfeld, J. F.: Gold Liposomes. In Proc 54th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, J. M. Corbett, R. V. W. Dimlich, J. R. Michael and N. J., Zaluzec (Eds.). San Francisco Press, San Francisco, CA, pp. 898-899 (1996).

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Fig.2. Darkfield STEM micrograph of unstained gold-lipid micelles (arrows), made from DPPE-gold, with diameters of ~ 14 nm. Fig. 3. Darkfield STEM micrograph of unstained gold-lipid bilayer, made from DPPE-gold; arrow points to single lipid layer region.

Gold Liposomes

Origin stories: Creating Nanogold® labeling for lipids

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Hainfeld, J. F.; Furuya, F. R., and Powell, R. D.: Metallosomes. J. Struct. Biol., 127, 152-160 (1999).

http://www.ncbi.nlm.nih.gov/pubmed/10527904
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Hirano K, Kinoshita T, Uemura T, Motohashi H, Watanabe Y, Ebihara T, Nishiyama H, Sato M, Suga M, Maruyama Y, Tsuji NM, Yamamoto M, Nishihara S, Sato C. Electron microscopy of primary cell cultures in solution and correlative optical microscopy using ASEM. Ultramicroscopy. 2014 Aug;143:52-66. doi: 10.1016/j.ultramic.2013.10.010. Epub 2013 Oct 22. PMID: 24216127.

https://pubmed.ncbi.nlm.nih.gov/24216127/
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Fig. 1 Microtubules, visualized by optical microscopy (OM) and atmospheric scanning electron microscopy (ASEM: inverted SEM). COS7 cells were labeled with anti-alpha-tubulin primary antibody followed by Alexa Fluor 488 FluoroNanogold™ secondary antibody (Nanoprobes). Top: Fluorescence microscopy image. Middle: ASEM image of the same cells after gold enhancement by GoldEnhance™ EM (Nanoprobes). Botton: Higher magnification image of the right filopodia. Microtubule rails are clearly observed in the cytoplasm. (Sato et al., 2013)

FluoroNanogold™: Correlative labeling with fluorescence and the atmospheric scanning electron microscope (ASEM)

A cutting-edge technique opens a window on the mechanics behind cellular dynamics, with the help of FluoroNanogold™, our combined label for both fluorescence and electron microscopy.

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