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Finely crafted nanoparticle products for imaging, microscopy and biodetection

All profits support our cancer research. In the course of our work, we have developed many useful nanoparticle technologies; we hope they are useful in your labs too. 

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    Results: 21 - 30 of 53 products
    Halo tag-Nanogold® custom conjugates! Available in 1.4, 5 and 12 nm - the missing link for Cryo-EM!
    new-solidDouble Gold Halo: Halo tag-Nanogold® 2-in-1 probe for Cryo-EM

    2-in-1 labeling for cryo-EM! Halo tags PLUS precision Nanogold® labels. The ultimate tool for labeling proteins in live cells, now available from Nanoprobes.

    EnzMet™ deposits silver at your HRP-labeled target, for sharp, clear staining.
    EnzMet HRP Stain for General Research Applications

    Crisp, archival HRP staining with precision EnzMet™, our metallographic substrate for horseradish peroxidase. Our most versatile formula, for wide-ranging research applications.

    HER2 staining in HER2-amplified tissue from a human breast cancer biopsy. Dr. Raymond R. Tubbs, Cleveland Clinic.
    EnzMet™: HRP Detection Kit for Immunohistochemistry & in situ Hybridization

    Clear, archival HRP staining with EnzMet™ metallographic substrate for horseradish peroxidase. Formulated for immunohistochemistry (IHC) and in situ hybridization (ISH) applications.

    Comparison of HRP detection with EnzMet™ vs. DAB and Metal-enhanced DAB Substrate Kit: 6xHis-tagged recombinant Src protein (Millipore # 14-326), probed using anti-His·Tag® mouse monoclonal antibody (Millipore # 70796-3) followed by goat anti-mouse IgG HRP conjugate (Millipore # 71045), then detected by: (left) DAB Substrate Kit,(center) metal-enhanced DAB Substrate Kit, and (right) EnzMet™ Western Blot HRP Detection Kit.
    EnzMet™: Western Blot HRP Detection Kit

    Upgrade from DAB! Crisp, black & archival, high-res, super-sensitive metallographic HRP substrate for Western blots with virtually no background.

    Left: Structure of Alexa Fluor® 488 and Nanogold® - Fab', showing covalent attachment of components. Right: Fluorescent staining obtained using combined combined Alexa Fluor® 488 and Nanogold® - Fab' tertiary probe. The specimen is a slide from the NOVA Lite ANA HEp-2 test, an indirect immunofluorescent test system for the screening and semi-quantitative determination of anti-nuclear antibodies (ANA) in human serum (see ). The slide was stained using positive pattern control human sera, a Mouse anti-Human secondary antiboidy, and combined Alexa Fluor® 488 and Nanogold® - Fab' tertiary probe. Specimens were washed with PBS (30 minutes) between each step, then blocked by the addition of 7 % nonfat dried milk to the tertiary antibody solution (original magnification 400 X).

    Precision Nanoparticle Secondaries

    Precision Nanogold® labels, covalently bound secondary antibodies, Fab’ fragments and streptavidin. Easily enhanced for any scope or blots!

    Alexa Fluor 647 FluoroNanogold™. 2-in-1 Labeling for Super-Res + TEM

    FluoroNanogold™ 2-in-1 Secondaries

    2-in-1 combined secondaries, with covalently bound Nanogold® PLUS fluorescent labels. Label once, and correlate easily between fluorescent context and EM detail.

    Left: Silver-enhanced Nanogold®-labeled K+ channel Kv2.1 subunit in rat brain, X15,000 (J.-H. Tao-Cheng, NIH). Right: Darkfield STEM micrographs of Maleimido Nanogold® labeled A β peptide targeting proteasomes; sample was stained with methylamine vanadate. White dots are 1.4 nm Nanogold®. From Gregori, L., Hainfeld, J.F., Simon, M.N., and Goldgaber, D. (1997). Binding of amyloid beta protein to the 20S proteasome. J. Biol. Chem. 272, 58-62.

    Nanogold® Secondaries

    Secondary antibodies, Fab’ fragments, and streptavidin, covalently bound for precise, permanent labeling with Nanogold®. Easily enhanced for any scope or blots!

    3-5 nm dodecanethiol functionalized gold (mean core diameter 4.08 ± 0.76 nm).
    Gold Nanomaterials: Functionalized Nanogold®

    Choose your solubility! Hydrophilic, amphiphilic, or hydrophobic nano materials, 3 or 5 nm functionalized gold nanoparticle labels.

    GoldEnhance nanoparticle developer for sharp, archival labeling for EM, LM and blots
    GoldEnhance™ Blots: Precision, Archival Developer for Western Blots

    Archival, precision staining for Western blots! High sensitivity, rapid enhancement.

    GoldEnhance nanoparticle developer for sharp, archival labeling for EM, LM and blots
    GoldEnhance™ EM Plus: SLOW Developer for Electron Microscopy

    SLOW nanoparticle developer with ultra-low background for electron microscopy. Easy timing for super-clear, precise results!

    GoldEnhance nanoparticle developer for sharp, archival labeling for EM, LM and blots
    GoldEnhance™ EM: Precision Developer for Electron Microscopy

    Rapid nanoparticle developer with high sensitivity for electron microscopy.

    GoldEnhance nanoparticle developer for sharp, archival labeling for EM, LM and blots
    GoldEnhance™ LM: Precision Developer for Light Microscopy

    Slow, precision nanoparticle developer with ultra-low background for light microscopy. See what you’ve been missing!