Updated: November 27, 2000
N A N O P R O B E S E - N E W S
Vol. 1, No. 1 November 27, 2000
This monthly newsletter is keep you informed about techniques to improve your immunogold labeling, highlight interesting articles and novel metal nanoparticle applications, and answer your questions. We hope you enjoy it and find it useful.
Have questions, or issues you would like to see addressed in the next
issue? Let us know by e-mailing tech@nanoprobes.com.
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Dr. Susan Cheng and co-workers at NINDS have refined their pre-embedding
procedure for the most uniform, consistent and reliable pre-embedding
labeling, using Nanogold®-Fab' and HQ Silver. Read their procedure in full
in the technical support section of our web site, or read their article
(Tanner, V. A., et al., J. Histochem. Cytochem., 44, 1481 (1996). Abstract: http://www.ncbi.nlm.nih.gov/pubmed/8985140).
Full details: www.nanoprobes.com/App3.html
More about Nanogold-Fab' conjugates: www.nanoprobes.com/NanoAb.html
More about HQ Silver: www.nanoprobes.com/Silver.html
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Did you know that Nanogold labeling with silver or gold enhancement is one
of the most sensitive and distinctive detection methods for in situ
hybridization? Nanogold-streptavidin easily accesses nuclear materials,
and upon autometallography - silver enhancement or gold enhancement
develops a crisp, clear black signal which is more sensitive than DAB,
EM-compatible and easily distinguished from other stains. See some of the results by Tubbs and co-workers in the American Journal of Pathology
(Zehbe, I., et al.: Am. J. Pathol., 150, 1553 (1997). Abstract: http://www.amjpathol.org/cgi/content/abstract/150/5/1553).
About Nanogold-ISH detection: www.nanoprobes.com/InSitu.html
More about Nanogold-Streptavidin: www.nanoprobes.com/Inf2016.html
More about gold enhancement: www.nanoprobes.com/GoldEnhance.html
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
We couldn't let our first issue out without reminding you of what makes Nanogold unique. Because it is covalently linked, you can label anything with a cross-linkable group. In their paper, Gregori and co-workers explain how they labeled a 39-residue peptide to study amyloid beta protein binding to the 20 proteasome (Gregori, et al., J. Biol. Chem., 272, 58 (1997)). They also took advantage of our unique NanoVan negative stain, which is specially formulated for use with Nanogold, to visualize the proteasome and determine the Nanogold location.
Full paper: http://www.jbc.org/cgi/reprint/272/1/58
More about NanoVan: www.nanoprobes.com/Nstain.html
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
We also make FluoroNanogold, a unique probe containing both Nanogold and fluorescein. Use it for correlative labeling, or for checking your labeling by fluorescence before EM processing. If you have any questions about this reagent, visit the technical support section of our web site to have them answered.
Our paper: http://www.jhc.org/cgi/reprint/45/7/947
Robinson and vandr's paper: http://www.jhc.org/cgi/reprint/45/5/631
More about FluoroNanogold: www.nanoprobes.com/FluoroN.html
Technical help for FluoroNanogold: www.nanoprobes.com/TechFN.html
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
If you want to use silver enhancement but can't because your specimens need physiological buffer, or you have problems with osmium etching, GoldEnhance can help. GoldEnhance works like silver enhancement, but instead deposits gold. Gold Enhanced particles are denser than silver enhanced ones for superior back-scattered electron (BSE) detection in the scanning electron microscope (SEM). Gold enhancement is impervious to osmium etching, and also features a longer time window from full development to the appearance of background, so it is easier to use in blotting and diagnostics applications. Learn more:
Our paper from M & M 99: www.nanoprobes.com/MSAGE99.html
Catalog Info and details: www.nanoprobes.com/GoldEnhance.html
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Larger gold particles offer surprising new uses. Researchers at UCSD have described how 40, 60 and 90 nm particles selectively scatter blue, green and red light respectively, forming the basis for new ways to detect biomolecules. This paper in PNAS explains how (Schultz, S., et al, Proc. Natl. Acad. Sci. USA, 97, 996 (2000)).
Reprint (pdf): http://www.pnas.org/cgi/reprint/97/3/996
The authors used silver enhancement of smaller colloidal gold particles in solution to prepare their large silver-on-gold particles. With our GoldEnhance gold-based enhancement reagent
( www.nanoprobes.com/GoldEnhance.html), you can have the option of making large gold particles instead.
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Robert Whetten and Grefory Schaaf report that gold clusters can show
intense optical activity; see Schaaff, T. Gregory; Whetten, Robert L., J. Phys. Chem. B, 104, 2630-2641 (2000).
Also, William Newcomb and co-workers use NanoVan negative stain to help determine the structure of viral capsids; see Sheaffer, A. K., Newcomb, W. W., et al., J. Virol., 74, 6838-48 (2000).
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
We are changing our line of colloidal gold probes to address new
applications, and making improvements to our manufacturing to give better stability and more reliable performance. We are currently looking for beta test sites who are in a position to try our prototype reagents in parallel with other colloidal gold probes. If you are interested, please let us know and tell us a little about your experiment (nano@nanoprobes.com). Requests will be reviewed for relevance to our development program.
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
To unsubscribe from this newsletter, simply reply to this message, and replace the subject line with "unsubscribe." Alternatively, visit our web site newsletter page at www.nanoprobes.com/Newsletter.html#unsubscribe. You will receive a confirmation via e-mail.
|
