Precision Nanogold® labels for Click chemistry!
Click chemistry is the reaction between an azide and an alkyne or similar group. It is a bio-orthogonal reaction (i.e. it does not occur naturally) which lets let you label selectively in living cells, tissues and even animals without cross-reactivity.
Three different reactivities are available to let you label both alkyne-modified and azide-modified targets using copper-catalyzed (CuAAC) or copper-free (SPAAC) reactions:
- 1.4 nm Mono-Azido Nanogold® reacts with alkynes and related reagents; may be used for copper-catalyzed (CuAAC) or copper-free (SPAAC) click reactions.
- 1.4 nm Mono-Alkyne Nanogold® reacts with azides via copper-catalyzed (CuAAC) reaction.
- 1.4 nm Mono-DBCO-Nanogold® reacts with azides via copper-free SPAAC reaction.
Applications:
- Label dynamic processes in living cells or tissues – Click reagents will not hydrolyze or degrade.
- Label components in living cells: do not react with endogenous species like biotin or cross-reactive proteins.
- High-resolution labeling of subunits or molecular sites: just incorporate the corresponding reactive azide or alkyne into the target – no antibody or probe required.
- Optical Visualization: use silver or gold enhancement to visualize by light microscopy or for super-sensitive blotting. See as little as 1 pg of target IgG!
- Multiplex your labeling: Add a click reaction to detect an additional target if there is no suitable antibody or probe.
1.4 nm Nanogold® Labeling Reagents
Nanogold® is a better gold label.
The 1.4 nm Nanogold® particle is a gold compound: it is not just adsorbed to proteins, like colloidal gold, but covalently reacts at specific sites under mild buffer conditions. This gives a well defined product that can be purified chromatographically.
Nanogold® brings the versatility of fluorescent conjugation to gold labeling.
Label virtually any molecule
Label any molecule with a suitable reactive group: oligonucleotides, lipids, peptides, proteins, enzyme inhibitors and others. This is a big improvement over colloidal gold, which may be adsorbed only to antibodies and a limited range of proteins and peptides.
Nanogold® is small and highly uniform in size, in sharp contrast to small colloidal gold preparations (most commonly used “1 nm” colloidal golds actually range from 1 to 3 nm).
Label your own biomolecules with our Nanogold® labeling reagents,
or check out our range of antibody IgG, Fab’ and streptavidin Conjugates with Nanogold®.
Left: Silver-enhanced Nanogold®-labeled K+ channel Kv2.1 subunit in rat brain, X15,000 (J.-H. Tao-Cheng, NIH).
Right: Darkfield STEM micrographs of Maleimido Nanogold® labeled A β peptide targeting proteasomes; sample was stained with methylamine vanadate. White dots are 1.4 nm Nanogold®.From Gregori, L., Hainfeld, J.F., Simon, M.N., and Goldgaber, D. (1997). Binding of amyloid beta protein to the 20S proteasome. J. Biol. Chem. 272, 58-62.
Easily enhanced for electron microscopy, light microscopy, cryo-EM, blots…
Try our precision nanoparticle developers for slow development with low background!
Features and Advantages
- Unparalleled penetration of conjugates -up to 40 µm!
- Higher density of immunolabeling than with larger gold probes.
- Can be conjugated to any molecule with a suitable reactive group. Available with different reactivities.
- Extremely uniform 1.4 nm gold particle.
- Label at specific sites which do not obstruct native reactivity.
- Close to stoichiometric labeling.
- Reacts under mild, neutral conditions.
- Conjugates are easily isolated by gel filtration.
- Conjugates are stable to a wide range of pH and ionic strengths.
- High stability: conjugates show unchanged reactivity after storage for a year.
