Precision Nanogold® labels for Click chemistry!
5 nm Click Nanogold® reagents are available with three different reactivities to let you label alkyne-modified or azide-modified targets, using either copper-catalyzed (CuAAC) or copper-free (SPAAC) reactions.
5 nm Alkyne Nanogold® reacts with azides via copper-catalyzed (CuAAC) reaction.
Applications:
- Label dynamic processes in living cells or tissues – Click reagents will not hydrolyze or degrade.
- Label components in living cells: no reaction with endogenous species or cross-reactive proteins.
- High-resolution precision labeling – no antibody or probe.
- Optical Visualization: use silver or gold enhancement to visualize by LM or for super-sensitive blotting.
- Multiplex your labeling: Use click reactions when no antibody or probe is available.
Reaction of 5 nm Alkyne-Nanogold® with (top) an azide via copper-catalyzed (CuAAC) click reaction.
5 nm Nanogold® Labeling Reagents
The precision and versatility of Nanogold®, now in a larger 5 nm size
Like our smaller 1.4 nm Nanogold® reagents, it covalently reacts at specific sites under mild buffer conditions. This gives a well defined product that can be purified chromatographically or by ammonium sulfate precipitation.
Unlike conventional colloidal gold, 5 nm Nanogold® does not require any additional macromolecules for stabilization. Conjugate probes are smaller than colloidal gold probes and achieve higher resolution, more precise and more nearly quantitative labeling.
Make any molecule a gold probe
Nanogold® brings the versatility of fluorescent conjugation to gold labeling. Now you have a choice of sizes for Nanogold®: 0.8, 1.4, 5 or 12 nm, enabling multiple labeling for electron microscopy without silver or gold enhancement!
- Antibodies
- Proteins
- Peptides
- Oligonucleotides and nucleosides: DNA, RNA and PNA
- Nanostructured materials
- Hormones
- Polymers
- Surfaces
Unconjugated 5 nm Nanogold®, showing monodisperse, highly regular, spherically symmetrical 5 nm gold core and uniform, thin and consistent ligand coating. This ensures a highly regular particle size, smaller probe size, and reliable, reproducible labeling and EM results.
Easily enhanced for electron microscopy, light microscopy, cryo-EM, blots…
Try our precision nanoparticle developers for easily controlled development with low background!
Features and Advantages
- Stable, soluble and biocompatible: Designer coating gives solubility and biocompatibility for in vivo use. No residual charge means minimal non-specific binding.
- Highly monodisperse. Size variation less than 15% for 5 nm gold. 1.4, 5 and 12 nm Nanogold® enable up to triple labeling with non-overlapping sizes
- Molecular precision: make smaller primary probes that get the gold closer to the target.
- Site-specific conjugation enables programmed incorporation into nanostructured materials or components of self-assembling systems.
- No need for additional proteins or polymers: smaller probes penetrate better and label more densely.
- Easily enhanced. Silver or gold enhancement enlarges while retaining monodispersity – or affords super-sensitive detection for blots and optical systems.
- Pick the optimum size: smaller for high resolution, molecular labeling, larger for visualization on a cellular scale.
Although larger than our 1.4 nm Nanogold®, our new 5 nm Nanogold® is still relatively small in size, and highly uniform. Because it does not require additional proteins for stabilization, 5 nm Nanogold® conjugates are smaller than most conjugates prepared with conventional colloidal gold and uniform in overall size, giving them faster and more consistent penetration into specimens.
If larger particle sizes are needed, 5 nm Nanogold® may easily be enhanced with GoldEnhance™ gold enhancement reagents, or our HQ Silver™ or LI Silver™ silver enhancement reagents: Depending on the reagents used, a brief exposure (1 – 4 minutes) produce highly visible grains 10-20 nm in size for electron microscope identification, while longer development times produce black signals, readily differentiated from organic chromogens, that are easily seen in the light microscope and on immunoblots, gels and Western blots.