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Head of a live mouse imaged by microCT following intravenous VivoVist™ administration, using a Bruker SkyScan 1276 at 13 um voxels resolution. 3D image generated and analyzed with Horos v4.0.0. Brain vessels of 50 microns or smaller were resolvable. Image kindly provided by Dr. Sean Marrelli, Department of Neurology at the McGovern Medical School at UT Health (Houston, TX).

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Fig.2. Darkfield STEM micrograph of unstained gold-lipid micelles (arrows), made from DPPE-gold, with diameters of ~ 14 nm. Fig. 3. Darkfield STEM micrograph of unstained gold-lipid bilayer, made from DPPE-gold; arrow points to single lipid layer region.

Gold Liposomes

Origin stories: Creating Nanogold® labeling for lipids

Fig. 1 Microtubules, visualized by optical microscopy (OM) and atmospheric scanning electron microscopy (ASEM: inverted SEM). COS7 cells were labeled with anti-alpha-tubulin primary antibody followed by Alexa Fluor 488 FluoroNanogold™ secondary antibody (Nanoprobes). Top: Fluorescence microscopy image. Middle: ASEM image of the same cells after gold enhancement by GoldEnhance™ EM (Nanoprobes). Botton: Higher magnification image of the right filopodia. Microtubule rails are clearly observed in the cytoplasm. (Sato et al., 2013)

FluoroNanogold™: Correlative labeling with fluorescence and the atmospheric scanning electron microscope (ASEM)

A cutting-edge technique opens a window on the mechanics behind cellular dynamics, with the help of FluoroNanogold™, our combined label for both fluorescence and electron microscopy.