Nanogold® Conjugates

Covalently bound, precision labels

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Nanogold® Conjugates

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FNG [FluoroNanogold™] is a probe containing two different markers; it opens the possibility of imaging the same sample at both the optical and the EM level. Thus, it is easy to perform a multimodal investigation, either on different cells or on the same cell visualized by correlative microscopy.

Custom Nanoparticle Synthesis & Nanoprobe Labels

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Left: Structure of Alexa Fluor® 488 and Nanogold® - Fab', showing covalent attachment of components. Right: Fluorescent staining obtained using combined combined Alexa Fluor® 488 and Nanogold® - Fab' tertiary probe. The specimen is a slide from the NOVA Lite ANA HEp-2 test, an indirect immunofluorescent test system for the screening and semi-quantitative determination of anti-nuclear antibodies (ANA) in human serum (see ). The slide was stained using positive pattern control human sera, a Mouse anti-Human secondary antiboidy, and combined Alexa Fluor® 488 and Nanogold® - Fab' tertiary probe. Specimens were washed with PBS (30 minutes) between each step, then blocked by the addition of 7 % nonfat dried milk to the tertiary antibody solution (original magnification 400 X).

Precision Nanoparticle Secondaries

Precision Nanogold® labels, covalently bound secondary antibodies, Fab’ fragments and streptavidin. Easily enhanced for any scope or blots!

Alexa Fluor 647 FluoroNanogold™. 2-in-1 Labeling for Super-Res + TEM

FluoroNanogold™ 2-in-1 Secondaries

2-in-1 combined secondaries, with covalently bound Nanogold® PLUS fluorescent labels. Label once, and correlate easily between fluorescent context and EM detail.

Left: Silver-enhanced Nanogold®-labeled K+ channel Kv2.1 subunit in rat brain, X15,000 (J.-H. Tao-Cheng, NIH). Right: Darkfield STEM micrographs of Maleimido Nanogold® labeled A β peptide targeting proteasomes; sample was stained with methylamine vanadate. White dots are 1.4 nm Nanogold®. From Gregori, L., Hainfeld, J.F., Simon, M.N., and Goldgaber, D. (1997). Binding of amyloid beta protein to the 20S proteasome. J. Biol. Chem. 272, 58-62.

Nanogold® Secondaries

Secondary antibodies, Fab’ fragments, and streptavidin, covalently bound for precise, permanent labeling with Nanogold®. Easily enhanced for any scope or blots!

Nanogold®-Lipid Conjugates: Each lipid molecule is covalently linked to a 0.8 nm or 1.4 nm Nanogold® cluster through its polar head group. Since the gold moiety is hydrophilic, these molecules retain the amphiphilic nature of the parent lipid, and behave like fatty acids or phospholipids.

Lipid-Nanogold® Precision Labels

The versatility of lipids, covalently labeled with Nanogold®. Study membranes by incorporation into the lipid phase, or gold-label micelles and vesicles so that they can be identified and visualized.

Precision Nanoparticle Labeling Products

Gold Nanoparticle Conjugates

FluoroNanogold

GoldEnhance™

GoldEnhance™

Nanogold® Conjugates

Antibodies & Streptavidin

Nanogold® Secondaries

FluoroNanogold™ Secondaries

Nanogold®-Lipids

Enhancers & Developers

GoldEnhance™

Silver Enhancers

HRP Stains

EnzMet™ HRP Stains

Nanogold® Labeling Reagents

Sulfo-NHS-Nanogold®

(Mono) Maleimido-Nanogold®

Click-Nanogold®

(Mono) Amino-Nanogold®

Charged Nanogold®

Non-Functionalized Nanogold®

Recombinant Protein Detection

Halo Tags with Nanogold®

Ni-NTA-Nanogold® for His Tags

GoldiBlot™ for His-tag Blots

MicroCT Contrast Agents

VivoVist™ Micro CT Contrast Agent

AuroVist™ Micro CT Contrast Agents

EnzMet™ HRP Stain for Micro CT

Gold Nanomaterials

Negative Stains for Electron Microscopy

Custom Synthesis available

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Nanogold® Conjugates

Enhancers & Developers

Nanogold® Labeling Reagents

HRP Stains

Gold Nanomaterials

MicroCT Contrast Agents

Recombinant Protein Detection

Negative Stains for Electron Microscopy

Finely crafted nanoparticle products for imaging, microscopy and biodetection

Nanogold® Conjugates

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Nanogold® Conjugates

Precision Nanoparticle Secondaries

FluoroNanogold™ 2-in-1 Secondaries

Nanogold® Secondaries