Technical Help: Negative Stains for EM

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See also

  • Guide to Gold Nanoparticle Labeling
    Detailed description of gold nanoparticle labeling, tips and tricks for successful conjugations, isolation of conjugates, and how to calculate labeling.



How do Negative Stains Work?

Do these stains work like osmication or uranyl acetate staining?

These reagents work only on cells or macromolecules in suspension: a layer of electron-dense material is left around the edges of the cell or macromolecule, and the edges are defined. The spaces between the structures of interest are filled in rather than the features themselves; this makes negative staining particularly appropriate for use with immunogold labeling. There is no positive staining of the biological specimen itself, unlike osmication.


Negative Stains: Catalog | Instructions | References


What concentration should I use?

Are these stains supplied at the right concentration for use, or should they be diluted further?

These stains are supplied as 2 % aqueous solutions. This is an appropriate concentration for use in most experiments. However, if lower concentrations are required, they may be diluted further with water.


Negative Stains: Catalog | Instructions | References


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