LI Silver Enhancement Kits: Precision Developer for EM, LM and Blots
Convenient and simple for EM, light microscopy or immunoblots, gels and Westerns. Ideal for use with Nanogold® reagents.
- High contrast signal for easy light microscope and immunoblot visibility.
- Lower background than other commercial developers.
- High sensitivity.
- Light Insensitive: observe development under normal lighting.
- Slow development (10-30 minutes) for precise monitoring.
- Compatible with all immunogold reagents.
- Best for light microscopy, gels and blots.
LI Silver Enhancement Kits: Precision Developer for EM, LM and Blots
Convenient and simple for EM, light microscopy or immunoblots, gels and Westerns. Ideal for use with Nanogold® reagents.
- High contrast signal for easy light microscope and immunoblot visibility.
- Lower background than other commercial developers.
- High sensitivity.
- Light Insensitive: observe development under normal lighting.
- Slow development (10-30 minutes) for precise monitoring.
- Compatible with all immunogold reagents.
- Best for light microscopy, gels and blots.
- 2013-250ML
LI Silver Enhancement Kits: Precision Developer for EM, LM and Blots
$ 162.00
Grow the size - Keep the precision
Combine nanoparticle developers with Nanogold® labels
- Nanogold® Labeling Reagents
available in 12 nm, 5 nm, 1.4 nm and 0.8 nm Nanogold® - Nanogold® Secondary Conjugates
with Antibody/Fab’/Streptavidin - FluoroNanogold™ 2-in-1 Secondaries
with Nanogold® PLUS fluorescence - Gold Nanomaterials
- Ni-NTA-Nanogold®
Product Information
LI Silver Enhancement Kits: Precision Developer for EM, LM and Blots
Precision Enhancement with Autometallography
Nanoparticle Silver Enhancement for EM, LM and Blots
How it works:
Metal particles can nucleate the highly specific deposition of silver from an appropriate silver salt solution in the presence of a suitable reducing agent. The silver coated gold particle then catalyzes more silver deposition and the silver grains grow in size.
-
Visualize even small gold particles
like Nanogold®
-
Enlarge up to 30 to 100 nm in size
-
Greatly enhanced visiblity in EM
-
Dark staining in the light microscope
-
Ultrasensitive visualization of antigens
on blots and in gels
Pairs beautifully with our precision Nanogold® labels:
- Nanogold® Labeling Reagents
available in 12 nm, 5 nm, 1.4 nm and 0.8 nm Nanogold® - Nanogold® Secondary Conjugates
with Antibody/Fab’/Streptavidin - FluoroNanogold™ 2-in-1 Secondaries
with Nanogold® PLUS fluorescence - Gold Nanomaterials
- Ni-NTA-Nanogold®
Our nanoparticle silver developer is deposited around gold nanoparticle labels, growing them for precise, clear visualization in electron microscopy, light microscopy, and western blots.
Grow the size - Keep the precision
Nanoparticle developers for nanoparticle labels
Unparalleled for EM!
Unique formulation for the best possible results with Nanogold® in critical applications, particularly in the electron microscope.
- Neutral pH for excellent preservation of structural integrity.
- Protective thickening agent for the most uniform, reproducible particle size distribution.
- Low background.
- Compatible with all immunogold reagents.
- Slightly light sensitive: should be used in indirect light or with a SafeLight.
- Best for electron microscopy.
For a comparison with other silver enhancers, see: Humbel, B. M., et al.: J. Histochem. Cytochem., 43, 735-737 (1995). For uses, see: Baude, A., et al.: Neuroscience, 69, 1031-1055 (1997)).
Convenient and simple for EM, light microscopy or immunoblots, gels and Westerns. Ideal for use with Nanogold® reagents.
- High contrast signal for easy light microscope and immunoblot visibility.
- Lower background than other commercial developers.
- High sensitivity.
- Light Insensitive: observe development under normal lighting.
- Slow development (10-30 minutes) for precise monitoring.
- Compatible with all immunogold reagents.
- Best for light microscopy, gels and blots.
Applications
Microscopy:
In electron microscopy the 1.4 nm Nanogold® develops to round grains that are more variable in size when < 20 nm. The development then slows, and the smaller grains “catch up.” At 80 nm, all silver grains from the 1.4 nm Nanogold® are quite uniform.
With HQ Silver™, this occurs after about a 3 minute development period, or with LI Silver after ^7 minutes. In the light microscope, superior penetration, low backgrounds, and ultrasensitive staining may be realized with Nanogold® reagents and LI Silver.
Immunoblots, Polyacrylamide Gels, Westerns and Nucleic Acid Detection:
Immunogold with silver enhancement provides simple, robust and permanent record assays with super sensitivity. Nanogold® can be grown in a reasonably controlled and uniform manner to produce particles ~2 to >100 nm in size with silver development times of ~30 seconds to ~30 minutes.
The smaller sizes are excellent for EM work whereas the intermediate sizes are useful for light and confocal microscopy. The largest sizes provide ultrasensitive visualization with the naked eye and are useful in immunoblots, gels and Westerns.
Nanogold®-anti-mouse Fab’ blotted against mouse IgG, developed with LI Silver (Nanoprobes). These ultra small gold particles nucleate silver deposition so well that unprecedented sensitivity is achieved. This immunodot blot shows 0.1 pg sensitivity (arrow).
Gel Staining for Nanogold®-Labeled Bands
After a protein or other molecule is labeled with Nanogold®, it may be run on a polyacrylamide gel. Subsequently, it may be developed with LI Silver to specifically stain the gold-containing bands.
The process is rapid, sensitive and selective, only taking a few minutes for dense staining to develop. It also works on transfer blots. This may be used to distinguish which components are labeled with Nanogold®.