John W. Dubendorff - Research Scientist

Dr. John Dubendorff examines cells in culture at the Nanoprobes lab.

John Dubendorff is an experienced molecular biologist/biochemist who brings an extensive knowledge of recombinant DNA technology and protein expression and function to projects at Nanoprobes. His experience includes spells as a Guest Researcher at the University of Vienna Biocenter (Vienna, Austria), research in the Department of Pathology at Stanford University, and postdoctoral research in the Biology Department (link) at Brookhaven National Laboratory and the Department of Microbiology at SUNY Stony Brook. He holds a Ph.D. in Chemistry from the University of Colorado, Boulder (1985), and M.S. (1978) and B.S. (1977) degrees in Chemistry from Emory University.

E-mail John at dubendorff@nanoprobes.com.

Publications

• Dubendorff, JW (1978). The interaction of aminoacyl-tRNA synthetases with mercurated active site-directed substrate analogs. Thesis for the degree of Master of Science in Chemistry, Emory University.

• Dubendorff, JW (1985). The interaction of base analog-substituted lambda PR promoters with E. coli RNA polymerase. Thesis for the degree of Doctor of Philosophy in Chemistry, University of Colorado.

• Caruthers, MH, LJ McBride, LP Bracco & JW Dubendorff (1985). Studies on nucleotide chemistry 15: Synthesis of oligodeoxynucleotides using amidine protected nucleosides. Nucleosides and Nucleotides 4: 95-105.

• Caruthers, MH, D Dellinger, K Prosser, AD Barone, JW Dubendorff, R Kierzek & M Rosendahl (1986). Nucleic acid synthesis and applications to molecular biology. Chemica Scripta 26: 25-30.

• Caruthers, MH, AD Barone, J Beltman, LP Bracco, DR Dodds, JW Dubendorff, SJ Eisenbeis, RB Gayle, K Prosser, MS Rosendahl, J Sutton & J-Y Tang (1986). The interaction of cro, cI and Escherichia coli RNA polymerase with operators and promoters. In: Protein Structure, Folding and Design Alan R. Liss, Inc., pp. 221-228.

• Dubendorff, JW, PL deHaseth, MS Rosendahl & MH Caruthers (1987). DNA functional groups required for formation of open complexes between E. coli RNA polymerase and the lambda PR promoter: Identification via base analog substitutions. Journal of Biological Chemistry 262: 892-898.
  • Abstract (courtesy of the Journal of Biological Chemistry).

• Studier, FW, AH Rosenberg, JJ Dunn & JW Dubendorff (1990). Use of T7 RNA polymerase to direct expression of cloned genes. Methods in Enzymology 185: 60-89.
  • Abstract (Medline).

• Dubendorff, JW & FW Studier (1991). Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. Journal of Molecular Biology 219: 45-59.
  • Abstract (Medline).

• Dubendorff, JW & FW Studier (1991). Creation of a bacteriophage T7 autogene: Cloning and expression of the gene for bacteriophage T7 RNA polymerase under control of its cognate promoter. Journal of Molecular Biology 219: 61-68.
  • Abstract (Medline).

• Dubendorff, JW, LJ Whittaker, JT Eltman & JS Lipsick (1992). Carboxy-terminal elements of c-Myb negatively regulate transcriptional activation in cis and in trans. Genes and Development 6: 2525-2535.
  • Abstract (Medline).

• Aziz, N, J Wu, JW Dubendorff, JS Lipsick, TW Sturgill & TP Bender (1993). c-Myb and v-Myb are differentially phosphorylated by p42mapk in vitro. Oncogene 8: 2259-2265.
  • Abstract (Medline).

• Dubendorff, JW (1996). Targeting linker insertion mutations to a region of interest. Methods in Molecular and Cellular Biology 5: 367-372.

• Dubendorff, JW & JS Lipsick (1999). Transcriptional regulation by the carboxyl terminus of c-Myb depends upon both the Myb DNA-binding domain and the DNA recognition site. Oncogene 18: 3452-3460.
  • Abstract (Medline).

• Wang, D-M, JW Dubendorff, CH Woo & JS Lipsick (1999). Functional analysis of carboxy-terminal deletion mutants of c-Myb. Journal of Virology 73: 5875-5886.
  • Abstract (courtesy of the Journal of Virology).
  • Reprint (PDF) (courtesy of the Journal of Virology).

Medline search: Dubendorff, J. W.